User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/02/11: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab: Spring 2014</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab: Spring 2014</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Objective == | ==Objective == | ||
* | * Image each treatment and standards. | ||
*If no treatment reverses fiber formation, fibers will be extracted from 50:1 and 110:1 ratios to expose them directly to each treatment. | *If no treatment reverses fiber formation, fibers will be extracted from 50:1 and 110:1 ratios to expose them directly to each treatment. | ||
*If a treatment reverses fiber formation, it will be investigated further in order to find the smallest amount of the variable that can be added to solution in order to cause fiber degradation. | *If a treatment reverses fiber formation, it will be investigated further in order to find the smallest amount of the variable that can be added to solution in order to cause fiber degradation. | ||
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===Actual pH for Treatments=== | ===Actual pH for Treatments=== | ||
[[Image:CHES_and_Phosphate_pH.png]] | [[Image:CHES_and_Phosphate_pH.png]] | ||
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[[Image:Controls.jpg|700px]] | [[Image:Controls.jpg|700px]] | ||
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* HEPES Desired pH 7; Looks similar to the controls. 40:1 appears less pink but doesn't appear to have visible fibers. 110:1 ratio much like CHES has become redder than the control. | * HEPES Desired pH 7; Looks similar to the controls. 40:1 appears less pink but doesn't appear to have visible fibers. 110:1 ratio much like CHES has become redder than the control. | ||
*Citric Acid Desired pH 3; Looks like the phosphate ratios. Has allowed AuNP's to fall out of solution to form fibers for all ratios. | *Citric Acid Desired pH 3; Looks like the phosphate ratios. Has allowed AuNP's to fall out of solution to form fibers for all ratios. | ||
* TRIS Desired pH 9; Looks like the CHES ratios. Looks like control. The only deviation is exhibited ion the 110:1 ratio in which the fibers appear redder. This is either due to more fibers being formed or could be the breakdown of the fibers due to the increased color of the solution. | * TRIS Desired pH 9; Looks like the CHES ratios. Looks like control. The only deviation is exhibited ion the 110:1 ratio in which the fibers appear redder. This is either due to more fibers being formed or could be the breakdown of the fibers due to the increased color of the solution. | ||
===Actual pH for Treatments=== | ===Actual pH for Treatments=== | ||
[[Image:HEPES_pH.png]] | [[Image:HEPES_pH.png]] | ||
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[[Image:Controls.jpg|700px]] | |||
[[Image:mes.png|700px|]] | |||
*MES pH 5; Caused all AuNP's to fall out of solution to form fibers. | |||
*EDTA; Looks very similar to the controls; however, the fibers became more red in the 50:1 with fibers and the 110:1. | |||
*EGTA; Looks very similar to the controls; however, the fibers became more red in the 50:1 with fibers and the 110:1. | |||
===Actual pH for Treatments=== | |||
[[Image:Mes1.png]] | |||
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[[Image:Controls.jpg|700px]] | |||
[[Image:22.png|700px|]] | |||
* 2,2 bipyridine caused all AuNP's to fall out of solution to form fibers. | |||
*Salicylic Acid wasn't that much different than the controls; however, the 50:1 with fibers and the 110:1 sample the fibers appeared more red. | |||
*Sodium Chloride caused all fibers to fall out of solution. The fibers weren't that abundant. | |||
===Actual pH for Treatments=== | |||
[[Image:2,2_bipyri.png]] | |||
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[[Image:Controls.jpg|700px]] | |||
[[Image:kcl.png|700px|]] | |||
**Note that the 50:1 ratio without fibers wasn't available for this test. | |||
*Potassium Chloride caused all fibers to fall out of solution. What is odd is the fibers don't seem numerous. | |||
*Calcium Chloride caused all fibers to fall out of solution. What is odd is the fibers don't seem numerous. | |||
*Magnesium Chloride caused all fibers to fall out of solution. What is odd is the fibers don't seem numerous. | |||
===Actual pH for Treatments=== | |||
[[Image:KCl.png]] | |||
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[[Image:Controls.jpg|700px]] | [[Image:Controls.jpg|700px]] | ||
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*Chloroform; Looks very similar to the controls. This samples was a little odd because we originally thought chloroform was water soluble. It isn't. As a result a separation of layers was observed, and the fibers wouldn't go into the chloroform layer. | *Chloroform; Looks very similar to the controls. This samples was a little odd because we originally thought chloroform was water soluble. It isn't. As a result a separation of layers was observed, and the fibers wouldn't go into the chloroform layer. | ||
*1,4 Dioxane' No change from the controls were observed. | *1,4 Dioxane' No change from the controls were observed. | ||
*Hydrochloric Acid allowed all AuNP's to fall out of solution to form fibers. | *Hydrochloric Acid allowed all AuNP's to fall out of solution to form fibers. | ||
===Actual pH for Treatments=== | ===Actual pH for Treatments=== | ||
[[Image:Chloroform1.png]] | [[Image:Chloroform1.png]] | ||
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[[Image:Controls.jpg|700px]] | [[Image:Controls.jpg|700px]] | ||
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*Acetone has no measurable differences from the controls. | *Acetone has no measurable differences from the controls. | ||
*SDS looked slightly different. In the 50:1 ratio with fibers and the 110:1 ratio the fibers appeared redder than the controls an more abundant. Perhaps this is the beginning of the fibers going back into solution? | *SDS looked slightly different. In the 50:1 ratio with fibers and the 110:1 ratio the fibers appeared redder than the controls an more abundant. Perhaps this is the beginning of the fibers going back into solution? | ||
===Actual pH for Treatments=== | ===Actual pH for Treatments=== | ||
[[Image:DMSO1.png]] | [[Image:DMSO1.png]] | ||
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==Notes== | ==Notes== |
Latest revision as of 23:43, 26 September 2017
Biomaterials Design Lab: Spring 2014 | Main project page Previous entry Next entry |
Objective
ProcedureCreating Stock Solutions of Gold, Lysozyme, and BSA
Synthesizing Lysozyme-AuNPs and BSA-AuNPs
Images
Actual pH for Treatments
Actual pH for Treatments
Actual pH for Treatments
Actual pH for Treatments
Actual pH for Treatments
Actual pH for Treatments
Actual pH for Treatments
Notes
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