User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/01/14: Difference between revisions
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*Salicylic Acid (Chelator) | *Salicylic Acid (Chelator) | ||
*2,2'-Bipyridyl (Chelator) | *2,2'-Bipyridyl (Chelator) | ||
*Possible Removal of Gold Ions Via Precipitation see attached article [[http://pubs.rsc.org/en/ | *Possible Removal of Gold Ions Via Precipitation see attached article [[http://pubs.rsc.org/en/Content/ArticleLanding/2012/GC/c2gc35222b#!divAbstract| here]] | ||
*To test whether or not any of these chelators are able to break up the fibers we will add 10 times as many particles of chelator per gold particle. This is to ensure the chelator is in excess to make sure an effect is seen. If one chelator does in fact cause the fibers to disappear we will decrease this ratio to see is a smaller amount of chelator will cause the same effect. | |||
===Manipulation of Temperature=== | ===Manipulation of Temperature=== | ||
*We know that fibers are present in solution at a temperature of 80 degrees Celsius after synthesis. | |||
* We don't know; however, if the fibers would still be stable if the temperatures were increased closer to boiling. | |||
* One problematic thing about increasing the temperature in order to see if the fibers can withstand greater heat stress is the chance of loosing solvent. Perhaps refluxing the solutions after synthesis would allow us to answer this question without loosing solvent. | |||
===Manipulation of pH=== | ===Manipulation of pH=== | ||
===Manipulation of Ionic Strength=== | * Test buffer solutions at pH 3, 5, 9, 11 to see effect on fibers. | ||
* If effect is seen, we will neutralize the solution back to pH 7 by bombarding the solution with acid or base. | |||
===Manipulation of Ionic Strength/ Add Different Cofactors=== | |||
*Ca+2 | |||
*Mg+2 | |||
*Na+1 | |||
*K+1 | |||
===Adding a new Water Miscible Solvent=== | ===Adding a new Water Miscible Solvent=== | ||
*Water has a dipole moment of 1.85D | |||
**Use Solvents with varying dipole moments that are still water miscible. | |||
***1,4 Dioxane- Dipole Moment .45D | |||
***Acetone- Dipole moment 2.88D | |||
***Dimethylformamide- Dipole moment 3.82D | |||
***Chloroform?- Dipole Moment 1.04D | |||
***DMSO- Dipole moment 3.96 | |||
===Manipulation of Light and Optics?=== | |||
*Microwave | |||
*IR | |||
*UV | |||
===Applying a Current?=== | |||
==Type of AuNP we plan to use to Test the Above Variables== | ==Type of AuNP we plan to use to Test the Above Variables== | ||
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*We will follow the same synthesis strategies as we did last semester; however, due to the large number of samples required for what we are proposing we will create many 1 ml aliquots for the 50 and 110 ratio of lysozyme linked AuNP's. | *We will follow the same synthesis strategies as we did last semester; however, due to the large number of samples required for what we are proposing we will create many 1 ml aliquots for the 50 and 110 ratio of lysozyme linked AuNP's. | ||
** Previous Lysozyme AuNP synthesis procedures we followed on [[User:Alexander_Cvitan/Notebook/Experimental_Biological_Chemistry_Lab/2013/10/30| Oct 30, 2013]] and [[User:Alexander_Cvitan/Notebook/Experimental_Biological_Chemistry_Lab/2013/11/06| Nov 6, 2013]]. | ** Previous Lysozyme AuNP synthesis procedures we followed on [[User:Alexander_Cvitan/Notebook/Experimental_Biological_Chemistry_Lab/2013/10/30| Oct 30, 2013]] and [[User:Alexander_Cvitan/Notebook/Experimental_Biological_Chemistry_Lab/2013/11/06| Nov 6, 2013]]. | ||
*Note that the number of gold particles present in the 1ml aliquots we plan on preparing is 1.505x10^17 particles. | |||
===More info on Lysozyme=== | ===More info on Lysozyme=== | ||
*The type of Lysozyme we used in lab came from here; | *The type of Lysozyme we used in lab came from here; | ||
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==If something Works How Do We Get Fibers Again?== | |||
*Source with peptide linked AuNP used transition metal ions in order to cause aggregation. Article Found [[http://onlinelibrary.wiley.com/doi/10.1002/smll.200700920/full| Here.]] | |||
__NOTOC__ | __NOTOC__ |
Revision as of 11:38, 15 January 2014
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2nd Semester Project IdeaOverarching Goal
Variables that are of InterestRemoval of Gold Ions[Chelation]
Manipulation of Temperature
Manipulation of pH
Manipulation of Ionic Strength/ Add Different Cofactors
Adding a new Water Miscible Solvent
Manipulation of Light and Optics?
Applying a Current?Type of AuNP we plan to use to Test the Above Variables
More info on Lysozyme
|
If something Works How Do We Get Fibers Again?
- Source with peptide linked AuNP used transition metal ions in order to cause aggregation. Article Found [Here.]