User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/01/14: Difference between revisions

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*EDTA (Chelator) [[http://www.sigmaaldrich.com/catalog/product/sial/e4884?lang=en&region=US| SIGMA]].
*EDTA (Chelator) [[http://www.sigmaaldrich.com/catalog/product/sial/e4884?lang=en&region=US| SIGMA]].
** If EDTA is able to break apart the fibers we would try and overwhelm the solution with additional gold ions to see if the fibers can be brought back.
** If EDTA is able to break apart the fibers we would try and overwhelm the solution with additional gold ions to see if the fibers can be brought back.
*Salicylic Acid (Chelator)


===Manipulation of Temperature===
===Manipulation of Temperature===

Revision as of 20:30, 14 January 2014

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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2nd Semester Project Idea

Overarching Goal

  • We want to try and see if we can take the AuNP fibers that we formed last semester and be able manipulate them. In doing so we want to see if we can make them disappear and reappear by altering ions present in the original solution, as well as through the addition of cofactors, manipulation of pH, manipulation of temperature, and the addition of various solvents.

Variables that are of Interest

Removal of Gold Ions

[Chelation]

  • EDTA (Chelator) [SIGMA].
    • If EDTA is able to break apart the fibers we would try and overwhelm the solution with additional gold ions to see if the fibers can be brought back.
  • Salicylic Acid (Chelator)

Manipulation of Temperature

Manipulation of pH

Manipulation of Ionic Strength

Adding a new Water Miscible Solvent

Type of AuNP we plan to use to Test the Above Variables

  • We know that Lysozyme linked AuNP's formed fibers at approximately the 45:1 Gold to Protein Ratio.
  • As a result we are going to create lysozyme linked AuNP at ratios 50:1 and 110:1 to see if our varying treatments have the same effect on budding fibers and well established fibers.
  • We will follow the same synthesis strategies as we did last semester; however, due to the large number of samples required for what we are proposing we will create many 1 ml aliquots for the 50 and 110 ratio of lysozyme linked AuNP's.

More info on Lysozyme


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