User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/10/23

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Objective

  • Follow the procedure as outlined by Dr.Hartings HERE.
  • Synthesize more HRP-Linked Au Nanoparticles at higher ratios.
  • Synthesize Myoglobin linked nanoparticles at standard ratios, dictated in yesterdays lab.
  • Run UV-Vis on HRP and Pepsin linked Au-NP's synthesized yesterday.


Procedure

Making HRP and Myoglobin AuNP's

  • In order to synthesize the HRP and Myoglobin nanoparticles, they were made as dictated by the table below.

  • After these solutions were made they were put in then oven at 80 degrees celsius for 4 hours.

UV-Vis of HRP and Pepsin AuNP's

Data