User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/10/16: Difference between revisions
From OpenWetWare
(10 intermediate revisions by the same user not shown) | |||
Line 30: | Line 30: | ||
===BSA Nanoparticle Synthesis=== | ===BSA Nanoparticle Synthesis=== | ||
====Procedure for Synthesis can be found [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/08/28|HERE]].==== | ====Procedure for Synthesis can be found [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/08/28|HERE]].==== | ||
*[HAuCl4] Stock- 2.33mM | |||
*[BSA] Stock- 15uM | |||
*Concentration of Gold must be 90 times the concentration of BSA. | |||
* In sum, we added 1.73ml of BSA and 1ml of HAuCl4 and diluted up to 10ml. This creates a molar ratio of 1:90 | |||
*Put in oven at 80 degrees Celsius for 3-4 hours. | |||
**Note the nanoparticles were placed in the fridge afterward to prevent the formation of nanoparticle fibers. | |||
===UV-Vis Runs=== | ===UV-Vis Runs=== | ||
Line 38: | Line 44: | ||
==Data== | ==Data== | ||
===HRP AU-NP's=== | |||
*This graph shows the catalytic activity of of HRP-linked AuNP's in relation to luminol. | |||
[[Image:Oct_16,_Exper_biochem_kinetics_HRPAUNP's.png]] | |||
*Based on the relatively static graph, or due to the lack in a shift in the spectra overtime shows that the HRP linked to the AuNP's is inactive. | |||
*This inactivity is most likely explained by the method of synthesis. If the heat was too high while preparing the HRP-linked nanoparticles it could have denatured the protein. | |||
[[Image:Oct_17_Abs_vs._time_@420nm.png]] | |||
*This graph represents the change in absorbance overtime at the wavelength where HRP is most active. Due to the fact that the graph in horizontal for all but the first point dictates that HRP most likely is inactive or its activity is interrupted by being linked to AuNP's. | |||
*The first point shows the solution at time zero before the HRP-AuNP's were added. As a result this big jump was to be expected. | |||
===UV-Vis Spectra of AU-NP's Currently Synthesized=== | |||
*The spectra represented below are going to be used to compare the different nanoparticles synthesized in lab. Note that the BSA nanoparticles aren't shown here because they had to be remade in lab last class due to the previous batch solidifying. | |||
[[Image:Oct_16_Exper_Biochem_UV-Vis_Spectra.png]] | |||
*Peak for Citrate AuNP's: 523nm | |||
*Peak for HRP-AuNP's: 526nm | |||
__NOTOC__ | __NOTOC__ |
Revision as of 08:28, 22 October 2013
Biomaterials Design Lab | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objective
ProcedureKinetics of HRP-AuNP'sSolutions Made
BSA Nanoparticle SynthesisProcedure for Synthesis can be found HERE.
UV-Vis Runs
DataHRP AU-NP's
UV-Vis Spectra of AU-NP's Currently Synthesized
|