User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/10/16: Difference between revisions

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** This reaction was then repeated using 1ml of HRP-AuNP's.   
** This reaction was then repeated using 1ml of HRP-AuNP's.   
*For both kinetic runs luminol and hydrogen peroxide were added together. HRP-AuNP's were then added to get the reaction to start. To ensure that both runs were the same volume, the volume of each run was normalized to 3ml using buffer.
*For both kinetic runs luminol and hydrogen peroxide were added together. HRP-AuNP's were then added to get the reaction to start. To ensure that both runs were the same volume, the volume of each run was normalized to 3ml using buffer.
===BSA Nanoparticle Synthesis===
===UV-Vis Runs===


==Data==
==Data==
__NOTOC__
__NOTOC__

Revision as of 11:33, 16 October 2013

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Objective

  • To complete the procedure outlined by Dr.Hartings HERE.
  • Run UV-Vis on Citrate, BSA, HRP-Au nanoparticles. Run a water blank.
  • Run kinetic analysis on the 60:1 diluted HRP-AU nanoparticles.
  • Remake BSA nanoparticles. (The BSA nanoparticles made previously formed fibers, rendering them useless).

Procedure

Kinetics of HRP-AuNP's

Solutions Made

  • Luminol
    • Stock was 1.46mM
    • A 20 times dilution was made to make a final solution volume of .072mM. (This was done in order to observe an abs on UV-Vis less than 1).
    • .15ml of Luminol was used for the reaction.
  • Hydrogen Peroxide
    • Stock was 44.29mM
    • Was diluted to be 50 times the concentration of the Hydrogen Peroxide. Thus the concentration is, 3.65mM.
    • .247ml of hydrogen peroxide was used for the reaction.
  • HRP-AuNP's
    • Originally 100ul of the 60:1 diluted sample was used. This solution was made using Au [] of .25mM and a HRP [] of 4.167uM. This caused an extremely slow kinetic reaction.
    • This reaction was then repeated using 1ml of HRP-AuNP's.
  • For both kinetic runs luminol and hydrogen peroxide were added together. HRP-AuNP's were then added to get the reaction to start. To ensure that both runs were the same volume, the volume of each run was normalized to 3ml using buffer.

BSA Nanoparticle Synthesis

UV-Vis Runs

Data
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