User:Ai Oikawa: Difference between revisions

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'''Office: 510 495 2462<br />
'''Office: 510 495 2462<br />
[mailto:AOikawa@lbl.gov AOikawa@lbl.gov]
[mailto:AOikawa@lbl.gov AOikawa@lbl.gov]
[http://openwetware.org/wiki/User:Ai_Oikawa/Notebook my note]
=='''''Expertise and Skills'''''==
*'''''DNA, RNA, and Protein analysis''''' -- DNA cloning and sequencing, RNA isolation and mRNA purification, RT-PCR, Northern blot, Protein extraction, Native-PAGE, SDS-PAGE, Western blotting, Protein and antibody purification using affinity chromatography
*'''''Lipid composition analysis''''' -- Lipid extraction, TLC analysis, Gas Chromatography
*'''''Histochemistry analysis''''' -- Histochemistry analysis In situ hybridization, Serial sectioning immunohistochemistry, Serial sectioning saccaride stains techniques
*'''''Intracellular localization''''' -- Immunofluorescence cytochemistry, Immnoelectoro-cytochemistory, Cell fractionation, Enzyme assay measurement
*'''''Microscopy''''' -- Light microscopy, Fluorescent microscopy, Electron microscopy
*'''''Protein-protein interaction''''' -- Affinity of protein expression in E.coli, GST-pull downs, BIA-CORE, Yeast two hybrid system
*'''''Gain or loss of function''''' -- Plant cell cultures, Agrobacterium transfection, Over-expression and RNAi, Floral dip methods in Arabidopsis, barley Virus-Induced Gene Silencing (by barley stripe mosaic virus)
*'''''Plant model systems''''' -- Arabidpsis, Rice, Barley, Monocot callus




=='''''Education and experience'''''==
* 2008-Current Postdoctoral Fellow: Joint BioEnergy Institute, California, USA
* 2005-2008 Ph.D: Iwate University, JAPAN
* 2007 Aug-Sep Visiting student: Yale University, Connecticut, USA
* 2003-2005 M.S: Iwate University, JAPAN
* 1998-2003 B.S: Iwate University, JAPAN


== '''''Awards'''''==


== '''''Awards and Grants recieved'''''==
'''2008'''  
'''2008'''  
*'''''[http://www.yamadazaidan.jp/english/overview.html The Yamada Science Foundation Japan]''''' (March 2008) I was awarded the Yamada science abroad research grant to study on “Unraveling the gene network involved in the construction of cell wall” in CA, USA.
*'''''[http://www.yamadazaidan.jp/english/overview.html The Yamada Science Foundation Japan]''''' (March 2008): I was awarded the Yamada science abroad research grant to study on “Unraveling the gene network involved in the construction of plant cell wall” (CA, USA).
 
'''2007'''  
'''2007'''  
*'''''[http://www.jspp.org/eng/12prize/index.html The Japanese Society of Plant Physiologists Fellow]''''' (July- Aug 2007) I was awarded the JSPP Fellow for the year 2007 to conduct a collaboration research with Dr. Timothy Nelson of Yale University (CT, USA) on "Unraveling the gene network involved in the barley seed development by LCM（Laser-captured microdissection)".
*'''''[http://www.jspp.org/eng/12prize/index.html The Japanese Society of Plant Physiologists Fellow]''''' (July- Aug 2007): I was selected for the JSPP Fellowship as a JSPP Fellow for the year 2007 to conduct collaboration　research with Dr. Timothy Nelson at Yale University (CT, USA) on “Unraveling the gene network involved in the barley seed development by LCM (Laser-captured microdissection)“.  
*'''''[http://www.lifesci-found.com/ The Life Science Foundation Japan (only japanese)]''''' (July 2007) I was awarded the life science travel grant to attend the Annual meeting of American Society of Plant Biologist (ASPB) -2007 held in Chicago, USA.
*'''''[http://www.lifesci-found.com/ The Life Science Foundation Japan (only japanese)]''''' (July 2007): I was awarded the life science travel grant to attend the Annual meeting of American Society of Plant Biologist (ASPB) held in Chicago, USA.  
 
 




=='''''Publications'''''==
=='''''Publications'''''==
'''2007'''
'''2007'''
*'''''Ai Oikawa''''', Abidur Rahman, Tetsuro Yamashita, Hideharu Taira and Shin-ichiro　Kidou. (2007) Virus-induced gene silencing of P23k in barley leaf reveals morphological changes involved in secondary wall formation. ''Journal of Experimental Botany 58:2617-2625''  [http://jxb.oxfordjournals.org/cgi/content/abstract/erm109v1 Paper (web)]
*'''''Ai Oikawa''''', Abidur Rahman, Tetsuro Yamashita, Hideharu Taira and Shin-ichiro　Kidou. (2007) Virus-induced gene silencing of P23k in barley leaf reveals morphological changes involved in secondary wall formation. ''Journal of Experimental Botany 58:2617-2625''  [http://jxb.oxfordjournals.org/cgi/content/abstract/erm109v1 Paper]
*'''''Ai Oikawa''''', Tetsuro Yamashita, Hideharu Taira, Shin-ichiro Ejiri and Shin-ichiro Kidou. (2007) Jasmonate-induced 23kD protein (JIP-23) is involved in seed development of barley. ''Plant Biotechnology 24:217-220''  [http://db1.wdc-jp.com/pdf_store/jspcmb/pdf/pb24_2/24_217.pdf  Paper (pdf)]
*'''''Ai Oikawa''''', Tetsuro Yamashita, Hideharu Taira, Shin-ichiro Ejiri and Shin-ichiro Kidou. (2007) Jasmonate-induced 23kD protein (JIP-23) is involved in seed development of barley. ''Plant Biotechnology 24:217-220''  [http://db1.wdc-jp.com/pdf_store/jspcmb/pdf/pb24_2/24_217.pdf  Paper]
 
'''2006'''
'''2006'''
*Shin-ichiro Kidou, '''''Ai Oikawa''''', Naoko Sasaki, Hiroshi Yasuda, Tetsuro Yamashita, Hiroyuki Koiwa, Kiyoaki Kato, Shin-ichiro Ejiri. (2006) Identification of a 23 KD protein (P23k) related to the sugar supply in germinating seed. ''Plant Biotechnology 23:357-364''  [http://www.jstage.jst.go.jp/article/plantbiotechnology/23/4/357/_pdf Paper (pdf)]
*Shin-ichiro Kidou, '''''Ai Oikawa''''', Naoko Sasaki, Hiroshi Yasuda, Tetsuro Yamashita, Hiroyuki Koiwa, Kiyoaki Kato, Shin-ichiro Ejiri. (2006) Identification of a 23 KD protein (P23k) related to the sugar supply in germinating seed. ''Plant Biotechnology 23:357-364''  [http://www.jstage.jst.go.jp/article/plantbiotechnology/23/4/357/_pdf Paper]
 
 




=='''''Posters presented in International meetings'''''==
=='''''Posters presented in International meetings'''''==
 
'''2008'''
* Naomi Geshi, '''''Ai Oikawa''''', Lan Yin, Mohammed Saddik Motawia, Henrik Vibe Scheller. Glycosyltranfserases involved in the plant cell wall polysaccharides biosynthesis.(2008) Annual Meeting of the Society for Glycobiology (Fort Worth, Texas)
* Henrik Vibe Scheller, '''''Ai Oikawa''''', Yuzuki Manabe, Jorunn Johansen, Herman Höfte, Mohammed Saddik Motawia, Naomi Geshi. (2008) Cell Wall Biosynthesis: Heterologous expression of Arabidopsis glycosyltransferases in Nicotiana benthamiana. XVI Congress of the Federation of European Societies of Plant Biology (Tampere, Finland)
'''2007'''
'''2007'''
*'''''Oikawa, A'''''., and Kidou, S. (2007) Functional analysis of the barley P23k protein by virus-induced gene silencing. The 5th international symposium of rice functional genomics (Tsukuba, Japan)
*'''''Ai Oikawa''''', and Shin-ichiro Kidou (2007). Functional analysis of the barley P23k protein by virus-induced gene silencing. The 5th international symposium of rice functional genomics (Tsukuba, Japan)
*'''''Oikawa, A'''''., Rahman A and  Kidou, S. (2007) Virus-induced gene silencing of P23k in barley leaf reveals morphological changes　involved in secondary wall formation. Plant Biology & Botany 2007 joint congress (Chicago, illinois, USA)
*'''''Ai Oikawa'''''., Abidur Rahman  and  Shin-ichiro Kidou(2007). Virus-induced gene silencing of P23k in barley leaf reveals morphological changes　involved in secondary wall formation. Plant Biology & Botany 2007 joint congress (Chicago, illinois, USA)
 
'''2006'''
'''2006'''
*'''''Oikawa, A'''''., Uemura A  and Kidou, S. (2006). Jasmonate-induced 23kD protein (JIP-23) is involved in seed development of barley. Iwate international Plant Science Symposium 2006 (Iwate, Japan)　   
*'''''Ai Oikawa''''', Aiko Uemura and Shin-ichiro Kidou (2006). Jasmonate-induced 23kD protein (JIP-23) is involved in seed development of barley. Iwate international Plant Science Symposium 2006 (Iwate, Japan)　   
*'''''Oikawa, A'''''.,  and Kidou, S. (2006). P23k relates to the sugar supply at various developmental stages in barley. 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress (Kyoto, Japan)  
*'''''Ai Oikawa''''',  and Shin-ichiro Kidou(2006). P23k relates to the sugar supply at various developmental stages in barley. 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress (Kyoto, Japan)  
*Shibasaki, K.,  '''''Oikawa, A'''''.  and Kidou, S. (2006). Identification of a novel barley 23kDa protein (P23k) related to the sugar supply in germinating seeds. 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress (Kyoto, Japan)
* Kyouhei Shibasaki, '''''Ai Oikawa''''' and Shin-ichiro Kidou (2006). Identification of a novel barley 23kDa protein (P23k) related to the sugar supply in germinating seeds. 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress (Kyoto, Japan)
 
'''2005'''
'''2005'''
*'''''Oikawa, A'''''., and Kidou, S. (2005) A barley 23kDa protein which is up-regulated by sugars at various developmental stages. 10th International Congress of SABRAO (Tsukuba, Japan)
*'''''Ai Oikawa''''', and Shin-ichiro Kidou (2005). A barley 23kDa protein which is up-regulated by sugars at various developmental stages. 10th International Congress of SABRAO (Tsukuba, Japan)
*'''''Oikawa, A'''''., and Kidou, S. (2005) Expression of a 23kDa protein is up-regulated by sugars at various developmental stages in barley.　The Gordon Research Conference (GRC) on Temperature Stress in Plants　(Ventura, California, USA)
*'''''Ai Oikawa''''', and Shin-ichiro Kidou (2005). Expression of a 23kDa protein is up-regulated by sugars at various developmental stages in barley.　The Gordon Research Conference (GRC) on Temperature Stress in Plants　(Ventura, California, USA)
 
 
 
 




=='''''Professional Affiliations'''''==
=='''''Professional Affiliations'''''==
* '''[http://www.aspb.org/  American Society of Plant Biologists]'''
* '''[http://www.aspb.org/  American Society of Plant Biologists]'''
* '''[http://www.jspp.org/eng/index.html  Japanese Society of Plant Physiologist]'''
* '''[http://www.jspp.org/eng/index.html  Japanese Society of Plant Physiologist]'''
* '''[http://www.spps.kvl.dk/cgi-bin/SPPS.pl  Scandinavian Plant Physiology Society]'''
* '''[http://www.spps.kvl.dk/cgi-bin/SPPS.pl  Scandinavian Plant Physiology Society]'''


=='''''Education'''''==


*Ph.D. (2008) Department of Biological Resource Science -- The United Graduate School of Agricultural Sciences, Iwate University, JAPAN.
=='''''Expertise and Skills'''''==
*M.S. (2005) Department of Biological Resource Science -- Graduate School of Agriculture, Master's Program, Iwate University,  JAPAN.
*DNA, RNA, and Protein analysis: DNA cloning and sequencing, RNA isolation and mRNA purification, RT-PCR, Northern blot, Protein extraction, Native-PAGE, SDS-PAGE, Western blotting, Protein and antibody purification using affinity chromatography, Enzyme assay measurement
*B.S. (2003) Department of Bioscience and Technology -- Faculty of Agriculture, Iwate University, JAPAN
*Histochemistry analysis: In situ hybridization, Serial sectioning immunohistochemistry, Serial sectioning saccharide stains techniques
 
*Intracellular localization: Immunofluorescence cytochemistry, Immnoelectoro-cytochemistory, Cell fractionation
=='''''Personal information'''''==
*Microscopy:Light microscopy, Fluorescent microscopy, Electron microscopy
 
*Protein-protein interaction: Affinity of protein expression in E.coli, GST-pull downs, BIA-CORE, Yeast two hybrid system
*Nationality -- Japanese
*Gain or loss of function: Plant cell cultures, Agrobacterium transfection, Over-expression and RNAi, Floral dip methods in Arabidopsis, Virus-induced gene silencing (by BSMV in monocots)
*Birth year -- 1980
*Plant model systems: Arabidopsis, Tobacco, Rice, Barley
*Place of Birth -- '''[http://en.wikipedia.org/wiki/Iwate_Prefecture  Iwate], [http://en.wikipedia.org/wiki/Japan  Japan]'''
**'''[http://en.wikipedia.org/wiki/Emishi  E-MI-SHI] - The original peoples in Iwate.'''
**'''[http://en.wikipedia.org/wiki/Kenji_Miyazawa  Kenji MIYAZAWA  (1896-1933)] - He is a scientist,  writer, painter, and religionist in Iwate.'''
**'''[http://en.wikipedia.org/wiki/Inazo_Nitobe Inazo NITOBE  (1862-1933)] - He is a Christian, agricultural economist, author, educator, diplomat, and politician from Iwate. He is also the author of [http://en.wikipedia.org/wiki/Bushido:_The_Soul_of_Japan ｢Bushido: The Soul of Japan｣].'''
 
==Useful links==
*[[OpenWetWare:Welcome|Introductory tutorial]]
*[[Help|OpenWetWare help pages]]

Revision as of 01:24, 5 February 2009

Ai Oikawa

Lawrence Berkeley National Laboratory
DOE Joint BioEnergy Institute
Buiding 978 Mailstop 4121, CA 94720
Room: 4450
 http://jbei.org/
Postdoctoral Fellow
Feedstocks Division
Office: 510 495 2462
 AOikawa@lbl.gov


Education and experience

  • 2008-Current Postdoctoral Fellow: Joint BioEnergy Institute, California, USA
  • 2005-2008 Ph.D: Iwate University, JAPAN
  • 2007 Aug-Sep Visiting student: Yale University, Connecticut, USA
  • 2003-2005 M.S: Iwate University, JAPAN
  • 1998-2003 B.S: Iwate University, JAPAN


Awards and Grants recieved

2008

  • The Yamada Science Foundation Japan (March 2008): I was awarded the Yamada science abroad research grant to study on “Unraveling the gene network involved in the construction of plant cell wall” (CA, USA).

2007

  • The Japanese Society of Plant Physiologists Fellow (July- Aug 2007): I was selected for the JSPP Fellowship as a JSPP Fellow for the year 2007 to conduct collaboration　research with Dr. Timothy Nelson at Yale University (CT, USA) on “Unraveling the gene network involved in the barley seed development by LCM (Laser-captured microdissection)“.
  • The Life Science Foundation Japan (only japanese) (July 2007): I was awarded the life science travel grant to attend the Annual meeting of American Society of Plant Biologist (ASPB) held in Chicago, USA.


Publications

2007

  • Ai Oikawa, Abidur Rahman, Tetsuro Yamashita, Hideharu Taira and Shin-ichiro　Kidou. (2007) Virus-induced gene silencing of P23k in barley leaf reveals morphological changes involved in secondary wall formation. Journal of Experimental Botany 58:2617-2625 Paper
  • Ai Oikawa, Tetsuro Yamashita, Hideharu Taira, Shin-ichiro Ejiri and Shin-ichiro Kidou. (2007) Jasmonate-induced 23kD protein (JIP-23) is involved in seed development of barley. Plant Biotechnology 24:217-220 Paper

2006

  • Shin-ichiro Kidou, Ai Oikawa, Naoko Sasaki, Hiroshi Yasuda, Tetsuro Yamashita, Hiroyuki Koiwa, Kiyoaki Kato, Shin-ichiro Ejiri. (2006) Identification of a 23 KD protein (P23k) related to the sugar supply in germinating seed. Plant Biotechnology 23:357-364 Paper


Posters presented in International meetings

2008

  • Naomi Geshi, Ai Oikawa, Lan Yin, Mohammed Saddik Motawia, Henrik Vibe Scheller. Glycosyltranfserases involved in the plant cell wall polysaccharides biosynthesis.(2008) Annual Meeting of the Society for Glycobiology (Fort Worth, Texas)
  • Henrik Vibe Scheller, Ai Oikawa, Yuzuki Manabe, Jorunn Johansen, Herman Höfte, Mohammed Saddik Motawia, Naomi Geshi. (2008) Cell Wall Biosynthesis: Heterologous expression of Arabidopsis glycosyltransferases in Nicotiana benthamiana. XVI Congress of the Federation of European Societies of Plant Biology (Tampere, Finland)

2007

  • Ai Oikawa, and Shin-ichiro Kidou (2007). Functional analysis of the barley P23k protein by virus-induced gene silencing. The 5th international symposium of rice functional genomics (Tsukuba, Japan)
  • Ai Oikawa., Abidur Rahman and Shin-ichiro Kidou(2007). Virus-induced gene silencing of P23k in barley leaf reveals morphological changes　involved in secondary wall formation. Plant Biology & Botany 2007 joint congress (Chicago, illinois, USA)

2006

  • Ai Oikawa, Aiko Uemura and Shin-ichiro Kidou (2006). Jasmonate-induced 23kD protein (JIP-23) is involved in seed development of barley. Iwate international Plant Science Symposium 2006 (Iwate, Japan)　
  • Ai Oikawa, and Shin-ichiro Kidou(2006). P23k relates to the sugar supply at various developmental stages in barley. 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress (Kyoto, Japan)
  • Kyouhei Shibasaki, Ai Oikawa and Shin-ichiro Kidou (2006). Identification of a novel barley 23kDa protein (P23k) related to the sugar supply in germinating seeds. 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress (Kyoto, Japan)

2005

  • Ai Oikawa, and Shin-ichiro Kidou (2005). A barley 23kDa protein which is up-regulated by sugars at various developmental stages. 10th International Congress of SABRAO (Tsukuba, Japan)
  • Ai Oikawa, and Shin-ichiro Kidou (2005). Expression of a 23kDa protein is up-regulated by sugars at various developmental stages in barley.　The Gordon Research Conference (GRC) on Temperature Stress in Plants　(Ventura, California, USA)


Professional Affiliations


Expertise and Skills

  • DNA, RNA, and Protein analysis: DNA cloning and sequencing, RNA isolation and mRNA purification, RT-PCR, Northern blot, Protein extraction, Native-PAGE, SDS-PAGE, Western blotting, Protein and antibody purification using affinity chromatography, Enzyme assay measurement
  • Histochemistry analysis: In situ hybridization, Serial sectioning immunohistochemistry, Serial sectioning saccharide stains techniques
  • Intracellular localization: Immunofluorescence cytochemistry, Immnoelectoro-cytochemistory, Cell fractionation
  • Microscopy:Light microscopy, Fluorescent microscopy, Electron microscopy
  • Protein-protein interaction: Affinity of protein expression in E.coli, GST-pull downs, BIA-CORE, Yeast two hybrid system
  • Gain or loss of function: Plant cell cultures, Agrobacterium transfection, Over-expression and RNAi, Floral dip methods in Arabidopsis, Virus-induced gene silencing (by BSMV in monocots)
  • Plant model systems: Arabidopsis, Tobacco, Rice, Barley
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