Tween 20
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| - | + | [[Image:250px-Polysorbate 20.png|thumb|250px|Structure of Tween20, aka Polyoxyethylene (20) sorbitan monolaurate]] | |
| + | '''Tween''' (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses. | ||
| + | == Storage/Stability == | ||
| + | * 2 years shelf life according to this chemicals manufacturer [http://www.jyhychem.com/pages/pro201_en.htm] | ||
| + | * 2 years for 20x Tris-buffered saline/Tween20 according to this data sheet [http://search.funakoshi.co.jp/data/datasheet/LVC/TA-XXX-TT.pdf] | ||
| + | |||
| + | Based on the SIGMA Tween20 data sheet [http://www.sigmaaldrich.com/sigma/product%20information%20sheet/p5927pis.pdf] | ||
| + | * aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate | ||
| + | * autoclaving is not recommended without testing for changes in properties; TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. | ||
| + | * TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures | ||
| + | * polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid | ||
| + | * polysorbates may reduce the activity of many preservatives | ||
| + | * no plastic incompatabilities have been observed | ||
| + | |||
| + | == Comments == | ||
| + | * "I've recently been doing a series of experiments comparing the sensitivity of various ELISA protocols. One thing I have noticed, which is slightly annoying, is that on opening a new tin of skimmed milk (Marvel) and a <u>new bottle of tween-20 (BDH) there are big reductions in the background</u>. OK, so this may have been expected, but not to the extent that I have noticed. Both of the 'old' reagents had probably been opened less than 6 months ago." [http://www.bio.net/bionet/mm/diagnost/1997-May/001119.html] | ||
| + | |||
| + | == See also == | ||
| + | * [[TBST]] - tris-buffered saline with Tween | ||
| + | * [[PBS-T]] - phosphate-buffered saline with Tween | ||
| + | * [[Critical micelle concentration (CMC)]] | ||
| + | |||
| + | == External links == | ||
| + | * [http://en.wikipedia.org/wiki/Tween_20 Tween 20] on Wikipedia | ||
| + | * [http://en.wikipedia.org/wiki/Tween_80 Tween 80] on Wikipedia | ||
[[Category:Material]] | [[Category:Material]] | ||
[[Category:Detergents]] | [[Category:Detergents]] | ||
Current revision
Tween (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses.
Contents |
Storage/Stability
- 2 years shelf life according to this chemicals manufacturer [1]
- 2 years for 20x Tris-buffered saline/Tween20 according to this data sheet [2]
Based on the SIGMA Tween20 data sheet [3]
- aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate
- autoclaving is not recommended without testing for changes in properties; TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions.
- TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures
- polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid
- polysorbates may reduce the activity of many preservatives
- no plastic incompatabilities have been observed
Comments
- "I've recently been doing a series of experiments comparing the sensitivity of various ELISA protocols. One thing I have noticed, which is slightly annoying, is that on opening a new tin of skimmed milk (Marvel) and a new bottle of tween-20 (BDH) there are big reductions in the background. OK, so this may have been expected, but not to the extent that I have noticed. Both of the 'old' reagents had probably been opened less than 6 months ago." [4]
See also
- TBST - tris-buffered saline with Tween
- PBS-T - phosphate-buffered saline with Tween
- Critical micelle concentration (CMC)
