Tween 20

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[[Image:250px-Polysorbate 20.png|thumb|250px|Structure of Tween20, aka Polyoxyethylene (20) sorbitan monolaurate]]
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'''Tween''' (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses.
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== Storage/Stability ==
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* 2 years shelf life according to this chemicals manufacturer [http://www.jyhychem.com/pages/pro201_en.htm]
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* 2 years for 20x Tris-buffered saline/Tween20 according to this data sheet [http://search.funakoshi.co.jp/data/datasheet/LVC/TA-XXX-TT.pdf]
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Based on the SIGMA Tween20 data sheet [http://www.sigmaaldrich.com/sigma/product%20information%20sheet/p5927pis.pdf]
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* aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate
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* autoclaving is not recommended without testing for changes in properties; TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions.
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* TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures
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* polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid
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* polysorbates may reduce the activity of many preservatives
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* no plastic incompatabilities have been observed
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== Comments ==
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* "I've recently been doing a series of experiments comparing the sensitivity of various ELISA protocols. One thing I have noticed, which is slightly annoying, is that on opening a new tin of skimmed milk (Marvel) and a <u>new bottle of tween-20 (BDH) there are big reductions in the background</u>. OK, so this may have been expected, but not to the extent that I have noticed. Both of the 'old' reagents had probably been opened less than 6 months ago." [http://www.bio.net/bionet/mm/diagnost/1997-May/001119.html]
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== See also ==
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* [[TBST]] - tris-buffered saline with Tween
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* [[PBS-T]] - phosphate-buffered saline with Tween
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* [[Critical micelle concentration (CMC)]]
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== External links ==
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* [http://en.wikipedia.org/wiki/Tween_20 Tween 20] on Wikipedia
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* [http://en.wikipedia.org/wiki/Tween_80 Tween 80] on Wikipedia
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[[Category:Material]]
[[Category:Material]]
[[Category:Detergents]]
[[Category:Detergents]]

Current revision

Structure of Tween20, aka Polyoxyethylene (20) sorbitan monolaurate
Structure of Tween20, aka Polyoxyethylene (20) sorbitan monolaurate

Tween (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses.

Contents

Storage/Stability

  • 2 years shelf life according to this chemicals manufacturer [1]
  • 2 years for 20x Tris-buffered saline/Tween20 according to this data sheet [2]

Based on the SIGMA Tween20 data sheet [3]

  • aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate
  • autoclaving is not recommended without testing for changes in properties; TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions.
  • TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures
  • polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid
  • polysorbates may reduce the activity of many preservatives
  • no plastic incompatabilities have been observed

Comments

  • "I've recently been doing a series of experiments comparing the sensitivity of various ELISA protocols. One thing I have noticed, which is slightly annoying, is that on opening a new tin of skimmed milk (Marvel) and a new bottle of tween-20 (BDH) there are big reductions in the background. OK, so this may have been expected, but not to the extent that I have noticed. Both of the 'old' reagents had probably been opened less than 6 months ago." [4]

See also

External links

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