Tween 20
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* TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures | * TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures | ||
* polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid | * polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid | ||
| - | * polysorbates may reduce the activity of many preservatives | + | * polysorbates may reduce the activity of many preservatives |
| + | * no plastic incompatabilities have been observed | ||
== Comments == | == Comments == | ||
Revision as of 14:02, 14 October 2008
Tween (full name Tween 20 or Polysorbate 20) is a common detergent used in biology used in cell lysis and membrane protein solubilisation among other uses.
Contents |
Storage/Stability
- 2 years shelf life according to this chemicals manufacturer [1]
- 2 years for 20x Tris-buffered saline/Tween20 according to this data sheet [2]
Based on the SIGMA Tween20 data sheet [3]
- aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate
- autoclaving is not recommended without testing for changes in properties; TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions.
- TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures
- polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid
- polysorbates may reduce the activity of many preservatives
- no plastic incompatabilities have been observed
Comments
- "I've recently been doing a series of experiments comparing the sensitivity of various ELISA protocols. One thing I have noticed, which is slightly annoying, is that on opening a new tin of skimmed milk (Marvel) and a new bottle of tween-20 (BDH) there are big reductions in the background. OK, so this may have been expected, but not to the extent that I have noticed. Both of the 'old' reagents had probably been opened less than 6 months ago." [4]
