Triton X-100

Structure of Triton X-100

Triton (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.

Storage/Stability

from the Pierce instructions manual [1]:

• store at room temperature protected from bright light
• susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. A
• addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated

hydroxytoluene per 500 moles of detergent to prevent autoxidation

See also

• Triton in protein solubilisation - Drummond:Solubility, Knight:Protein solubility
• .. in antibody washing/blocking - Silver:Immunofluorescence
• .. as part of miniprep - Miniprep/Qiagen kit
• .. in cell lysis and protein solubilisation for Western blot - Silver: Lysate for Western

External links

• Triton at the Wikipedia