Triton X-100 - Revision history

Etienne Robillard at 14:25, 24 January 2013

2013-01-24T14:25:57Z

←Older revision		Revision as of 14:25, 24 January 2013
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	'''Triton''' (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.		'''Triton''' (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.

-	== ~~Storage/Stability~~ ==	+	== Usage ==
	* Recommended shelf life: 2 years [http://www.romil.com/Shelf_Life_SPS.pdf]		* Recommended shelf life: 2 years [http://www.romil.com/Shelf_Life_SPS.pdf]
	* Keep at room temperature protected from bright light		* Keep at room temperature protected from bright light

Etienne Robillard: /* Storage/Stability */

2013-01-24T14:25:08Z

Storage/Stability

←Older revision		Revision as of 14:25, 24 January 2013
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	== Storage/Stability ==		== Storage/Stability ==
-	* ~~recommended~~ shelf life: 2 years [http://www.romil.com/Shelf_Life_SPS.pdf]	+	* Recommended shelf life: 2 years [http://www.romil.com/Shelf_Life_SPS.pdf]
-		+	* Keep at room temperature protected from bright light
-		+	* Susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas.
-	~~from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]:~~	+	* Addition of [[EDTA]] and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated hydroxytoluene per 500 moles of detergent to prevent autooxidation.
-	* ~~store~~ at room temperature protected from bright light	+	** [[User:Etienne Robillard\|Etienne Robillard]] 09:25, 24 January 2013 (EST) Hence, adding EDTA to Triton-X100 may also be used for preventing Mg2+ dependent (ie: NMDA) ligand oxidation. Thus proper precautions must be taken when handling Triton X-100/EDTA reagents. (safety google, gas mask, etc)
-	* ~~susceptible~~ to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas.	+
-	* ~~addition~~ of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated	+
-	hydroxytoluene per 500 moles of detergent to prevent ~~autoxidation~~	+

	== Tips ==		== Tips ==

Jakob Suckale: /* See also */

2009-07-29T20:04:51Z

Jakob Suckale: Triton moved to Triton X-100 over redirect: more precise name

2009-07-28T13:23:11Z

Triton moved to Triton X-100 over redirect: more precise name

←Older revision

Revision as of 13:23, 28 July 2009

Jakob Suckale: /* Storage/Stability */

2008-10-14T18:36:15Z

Storage/Stability

←Older revision		Revision as of 18:36, 14 October 2008
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	== Storage/Stability ==		== Storage/Stability ==
	* recommended shelf life: 2 years [http://www.romil.com/Shelf_Life_SPS.pdf]		* recommended shelf life: 2 years [http://www.romil.com/Shelf_Life_SPS.pdf]
		+

	from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]:		from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]:

Jakob Suckale: /* Storage/Stability */

2008-10-14T18:36:03Z

Storage/Stability

←Older revision		Revision as of 18:36, 14 October 2008
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	== Storage/Stability ==		== Storage/Stability ==
		+	* recommended shelf life: 2 years [http://www.romil.com/Shelf_Life_SPS.pdf]
		+
	from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]:		from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]:
	* store at room temperature protected from bright light		* store at room temperature protected from bright light
	* susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas.		* susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas.
	* addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated		* addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated
-	hydroxytoluene per 500 moles of detergent to prevent autoxidation	+	hydroxytoluene per 500 moles of detergent to prevent autoxidation

	== Tips ==		== Tips ==

Jakob Suckale: tips

2008-10-14T18:32:38Z

tips

←Older revision		Revision as of 18:32, 14 October 2008
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	from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]:		from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]:
	* store at room temperature protected from bright light		* store at room temperature protected from bright light
-	* susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. A	+	* susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas.
	* addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated		* addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated
	hydroxytoluene per 500 moles of detergent to prevent autoxidation		hydroxytoluene per 500 moles of detergent to prevent autoxidation
-		+
		+	== Tips ==
		+	from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]:
		+	* The presence of the phenyl group of Triton X-100 absorbs strongly at 280 nm. Consequently, absorbance at 280 nm
		+	has to be adjusted when estimating protein concentration of samples solubilized using Triton X-100
		+	* When used at concentrations greater than 0.015%, Triton X-100 will exist in micelles having MW >90,000. In this
		+	form, the detergent cannot be removed by traditional dialysis or gel filtration. Use a removing gel in this case.
		+
	== See also ==		== See also ==
	* Triton in protein solubilisation - [[Drummond:Solubility]], [[Knight:Protein solubility]]		* Triton in protein solubilisation - [[Drummond:Solubility]], [[Knight:Protein solubility]]

Jakob Suckale: storage/stability

2008-10-14T18:32:11Z

storage/stability

←Older revision		Revision as of 18:32, 14 October 2008
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	[[Image:200px-Triton X-100.png\|thumb\|200px\|Structure of Triton X-100]]		[[Image:200px-Triton X-100.png\|thumb\|200px\|Structure of Triton X-100]]
-

	'''Triton''' (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.		'''Triton''' (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.

-		+	== Storage/Stability ==
		+	from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]:
		+	* store at room temperature protected from bright light
		+	* susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. A
		+	* addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated
		+	hydroxytoluene per 500 moles of detergent to prevent autoxidation
		+
	== See also ==		== See also ==
	* Triton in protein solubilisation - [[Drummond:Solubility]], [[Knight:Protein solubility]]		* Triton in protein solubilisation - [[Drummond:Solubility]], [[Knight:Protein solubility]]

Jakob Suckale: illustration

2008-10-14T18:22:21Z

illustration

←Older revision		Revision as of 18:22, 14 October 2008
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-	'''Triton''' (full name Triton X-100) is a common detergent used for solubilisation, permeabilisation, and lysis.	+	[[Image:200px-Triton X-100.png\|thumb\|200px\|Structure of Triton X-100]]
		+
		+
		+	'''Triton''' (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.

Jakob Suckale: external links

2008-10-14T18:18:11Z

external links

←Older revision		Revision as of 18:18, 14 October 2008
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	* .. as part of miniprep - [[Miniprep/Qiagen kit]]		* .. as part of miniprep - [[Miniprep/Qiagen kit]]
	* .. in cell lysis and protein solubilisation for Western blot - [[Silver: Lysate for Western]]		* .. in cell lysis and protein solubilisation for Western blot - [[Silver: Lysate for Western]]
		+
		+	== External links ==
		+	* [http://en.wikipedia.org/wiki/Triton_X-100 Triton at the Wikipedia]

	[[Category:Material]]		[[Category:Material]]
	[[Category:Detergents]]		[[Category:Detergents]]

←Older revision		Revision as of 20:04, 29 July 2009
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	* .. as part of miniprep - [[Miniprep/Qiagen kit]]		* .. as part of miniprep - [[Miniprep/Qiagen kit]]
	* .. in cell lysis and protein solubilisation for Western blot - [[Silver: Lysate for Western]]		* .. in cell lysis and protein solubilisation for Western blot - [[Silver: Lysate for Western]]
		+	* [[Critical micelle concentration (CMC)]]

	== External links ==		== External links ==