Triton X-100: Difference between revisions
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* Keep at room temperature protected from bright light | * Keep at room temperature protected from bright light | ||
* Susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. | * Susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. | ||
* Addition of [[EDTA]] and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated hydroxytoluene per 500 moles of detergent to prevent autooxidation. | * Addition of [[EDTA]] and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of [[BHT|butylated hydroxytoluene]] per 500 moles of detergent to prevent autooxidation. | ||
== Tips == | == Tips == | ||
Latest revision as of 04:47, 28 June 2013
Triton (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.
Usage
- Recommended shelf life: 2 years [1]
- Keep at room temperature protected from bright light
- Susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas.
- Addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated hydroxytoluene per 500 moles of detergent to prevent autooxidation.
Tips
from the Pierce instructions manual [2]:
- The presence of the phenyl group of Triton X-100 absorbs strongly at 280 nm. Consequently, absorbance at 280 nm
has to be adjusted when estimating protein concentration of samples solubilized using Triton X-100
- When used at concentrations greater than 0.015%, Triton X-100 will exist in micelles having MW >90,000. In this
form, the detergent cannot be removed by traditional dialysis or gel filtration. Use a removing gel in this case.
See also
- Triton in protein solubilisation - Drummond:Solubility, Knight:Protein solubility
- .. in antibody washing/blocking - Silver:Immunofluorescence
- .. as part of miniprep - Miniprep/Qiagen kit
- .. in cell lysis and protein solubilisation for Western blot - Silver: Lysate for Western
- Critical micelle concentration (CMC)
