Triton X-100: Difference between revisions

Revision as of 11:32, 14 October 2008

Triton (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.

from the Pierce instructions manual [1]:

store at room temperature protected from bright light
susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. A
addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated

hydroxytoluene per 500 moles of detergent to prevent autoxidation

@@ Line 1: / Line 1: @@
 [[Image:200px-Triton X-100.png|thumb|200px|Structure of Triton X-100]]
 '''Triton''' (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.
+== Storage/Stability ==
+from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]:
+* store at room temperature protected from bright light
+* susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. A
+* addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated
+hydroxytoluene per 500 moles of detergent to prevent autoxidation
 == See also ==
 * Triton in protein solubilisation - [[Drummond:Solubility]], [[Knight:Protein solubility]]