Triton X-100

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'''Triton''' (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.
'''Triton''' (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.
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== Storage/Stability ==
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== Usage ==
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* Recommended shelf life: 2 years [http://www.romil.com/Shelf_Life_SPS.pdf]
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* Keep at room temperature protected from bright light
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* Susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas.
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* Addition of [[EDTA]] and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated hydroxytoluene per 500 moles of detergent to prevent autooxidation.
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** [[User:Etienne Robillard|Etienne Robillard]] 09:25, 24 January 2013 (EST) Hence, adding EDTA to Triton-X100 may also be used for preventing Mg2+ dependent (ie: NMDA) ligand oxidation. Thus proper precautions must be taken when handling Triton X-100/EDTA reagents. (safety google, gas mask, etc)
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== Tips ==
from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]:
from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]:
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* store at room temperature protected from bright light
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* The presence of the phenyl group of Triton X-100 absorbs strongly at 280 nm. Consequently, absorbance at 280 nm
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* susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. A
+
has to be adjusted when estimating protein concentration of samples solubilized using Triton X-100
-
* addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated
+
* When used at concentrations greater than 0.015%, Triton X-100 will exist in micelles having MW >90,000. In this
-
hydroxytoluene per 500 moles of detergent to prevent autoxidation
+
form, the detergent cannot be removed by traditional dialysis or gel filtration. Use a removing gel in this case.
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== See also ==
== See also ==
* Triton in protein solubilisation - [[Drummond:Solubility]], [[Knight:Protein solubility]]
* Triton in protein solubilisation - [[Drummond:Solubility]], [[Knight:Protein solubility]]
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* .. as part of miniprep - [[Miniprep/Qiagen kit]]
* .. as part of miniprep - [[Miniprep/Qiagen kit]]
* .. in cell lysis and protein solubilisation for Western blot - [[Silver: Lysate for Western]]
* .. in cell lysis and protein solubilisation for Western blot - [[Silver: Lysate for Western]]
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* [[Critical micelle concentration (CMC)]]
== External links ==
== External links ==

Revision as of 10:25, 24 January 2013

Structure of Triton X-100
Structure of Triton X-100

Triton (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.

Contents

Usage

  • Recommended shelf life: 2 years [1]
  • Keep at room temperature protected from bright light
  • Susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas.
  • Addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated hydroxytoluene per 500 moles of detergent to prevent autooxidation.
    • Etienne Robillard 09:25, 24 January 2013 (EST) Hence, adding EDTA to Triton-X100 may also be used for preventing Mg2+ dependent (ie: NMDA) ligand oxidation. Thus proper precautions must be taken when handling Triton X-100/EDTA reagents. (safety google, gas mask, etc)

Tips

from the Pierce instructions manual [2]:

  • The presence of the phenyl group of Triton X-100 absorbs strongly at 280 nm. Consequently, absorbance at 280 nm

has to be adjusted when estimating protein concentration of samples solubilized using Triton X-100

  • When used at concentrations greater than 0.015%, Triton X-100 will exist in micelles having MW >90,000. In this

form, the detergent cannot be removed by traditional dialysis or gel filtration. Use a removing gel in this case.

See also

External links

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