Triton X-100
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== Storage/Stability == | == Storage/Stability == | ||
| + | * recommended shelf life: 2 years [http://www.romil.com/Shelf_Life_SPS.pdf] | ||
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from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]: | from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]: | ||
* store at room temperature protected from bright light | * store at room temperature protected from bright light | ||
* susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. | * susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. | ||
* addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated | * addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated | ||
| - | hydroxytoluene per 500 moles of detergent to prevent autoxidation | + | hydroxytoluene per 500 moles of detergent to prevent autoxidation |
== Tips == | == Tips == | ||
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* .. as part of miniprep - [[Miniprep/Qiagen kit]] | * .. as part of miniprep - [[Miniprep/Qiagen kit]] | ||
* .. in cell lysis and protein solubilisation for Western blot - [[Silver: Lysate for Western]] | * .. in cell lysis and protein solubilisation for Western blot - [[Silver: Lysate for Western]] | ||
| + | * [[Critical micelle concentration (CMC)]] | ||
== External links == | == External links == | ||
Revision as of 16:04, 29 July 2009
Triton (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.
Contents |
Storage/Stability
- recommended shelf life: 2 years [1]
from the Pierce instructions manual [2]:
- store at room temperature protected from bright light
- susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas.
- addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated
hydroxytoluene per 500 moles of detergent to prevent autoxidation
Tips
from the Pierce instructions manual [3]:
- The presence of the phenyl group of Triton X-100 absorbs strongly at 280 nm. Consequently, absorbance at 280 nm
has to be adjusted when estimating protein concentration of samples solubilized using Triton X-100
- When used at concentrations greater than 0.015%, Triton X-100 will exist in micelles having MW >90,000. In this
form, the detergent cannot be removed by traditional dialysis or gel filtration. Use a removing gel in this case.
See also
- Triton in protein solubilisation - Drummond:Solubility, Knight:Protein solubility
- .. in antibody washing/blocking - Silver:Immunofluorescence
- .. as part of miniprep - Miniprep/Qiagen kit
- .. in cell lysis and protein solubilisation for Western blot - Silver: Lysate for Western
- Critical micelle concentration (CMC)
