Triton X-100

(Difference between revisions)

Revision as of 14:32, 14 October 2008

Structure of Triton X-100

Triton (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.

from the Pierce instructions manual [1]:

store at room temperature protected from bright light
susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas.
addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated

hydroxytoluene per 500 moles of detergent to prevent autoxidation

from the Pierce instructions manual [2]:

The presence of the phenyl group of Triton X-100 absorbs strongly at 280 nm. Consequently, absorbance at 280 nm

has to be adjusted when estimating protein concentration of samples solubilized using Triton X-100

When used at concentrations greater than 0.015%, Triton X-100 will exist in micelles having MW >90,000. In this

form, the detergent cannot be removed by traditional dialysis or gel filtration. Use a removing gel in this case.

@@ Line 6: / Line 6: @@
 from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]:
 * store at room temperature protected from bright light
-* susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. A
+* susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas.
 * addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated
 hydroxytoluene per 500 moles of detergent to prevent autoxidation
+== Tips ==
+from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]:
+* The presence of the phenyl group of Triton X-100 absorbs strongly at 280 nm. Consequently, absorbance at 280 nm
+has to be adjusted when estimating protein concentration of samples solubilized using Triton X-100
+* When used at concentrations greater than 0.015%, Triton X-100 will exist in micelles having MW >90,000. In this
+form, the detergent cannot be removed by traditional dialysis or gel filtration. Use a removing gel in this case.
 == See also ==
 * Triton in protein solubilisation - [[Drummond:Solubility]], [[Knight:Protein solubility]]