Triton X-100: Difference between revisions
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[[Image:200px-Triton X-100.png|thumb|200px|Structure of Triton X-100]] | [[Image:200px-Triton X-100.png|thumb|200px|Structure of Triton X-100]] | ||
'''Triton''' (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis. | '''Triton''' (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis. | ||
== Storage/Stability == | |||
from the Pierce instructions manual [http://www.piercenet.com/files/0084dh4.pdf]: | |||
* store at room temperature protected from bright light | |||
* susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. A | |||
* addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated | |||
hydroxytoluene per 500 moles of detergent to prevent autoxidation | |||
== See also == | == See also == | ||
* Triton in protein solubilisation - [[Drummond:Solubility]], [[Knight:Protein solubility]] | * Triton in protein solubilisation - [[Drummond:Solubility]], [[Knight:Protein solubility]] |
Revision as of 11:32, 14 October 2008
Triton (full name Triton X-100) is a common, nonionic detergent used for solubilisation, permeabilisation, and lysis.
Storage/Stability
from the Pierce instructions manual [1]:
- store at room temperature protected from bright light
- susceptible to autoxidation upon exposure to air. After opening, store the unused solution sealed in an air-tight vial containing minimal air space or from which the air has been purged with nitrogen gas. A
- addition of EDTA and butylated hydroxytoluene will help to prevent oxidation. Use 1 mole of butylated
hydroxytoluene per 500 moles of detergent to prevent autoxidation
See also
- Triton in protein solubilisation - Drummond:Solubility, Knight:Protein solubility
- .. in antibody washing/blocking - Silver:Immunofluorescence
- .. as part of miniprep - Miniprep/Qiagen kit
- .. in cell lysis and protein solubilisation for Western blot - Silver: Lysate for Western