Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C4H11NO3).
- The formula weight is 121.14
- The pKa of Tris is 8.3
- dpKa/dT is -.028
- A buffer at pH 6.8 at 25°C will have a pH of 6.46 at 37°C
- Note that adding HCl during titration will affect the temperature of the solution
- Dilution by a factor of 10 will reduce the pH by 0.1 pH units.