Tris - Revision history

Jakob Suckale: /* See also */

2008-10-14T17:17:20Z

2008-10-14T17:17:04Z

2008-10-14T17:12:36Z

Stability

←Older revision		Revision as of 17:12, 14 October 2008
Line 23:		Line 23:
	== Stability ==		== Stability ==
	* 18 months for Tris-HCl buffer according to this data sheet [http://www.labvision.com/pdf/uvdatasheet/AP-9005-XXX.pdf]		* 18 months for Tris-HCl buffer according to this data sheet [http://www.labvision.com/pdf/uvdatasheet/AP-9005-XXX.pdf]
-	* 2 ~~yeasrs~~ for Tris-EDTA according to this data sheet [http://www.labtech.co.uk/downloads/biothema/21-500.pdf]	+	* 2 years for Tris-EDTA according to this data sheet [http://www.labtech.co.uk/downloads/biothema/21-500.pdf]


-	[[Category:Material]] ~~and~~ [[Category:Buffers]]	+	[[Category:Material]]
		+	[[Category:Buffers]]

2008-10-14T17:12:10Z

stability

←Older revision		Revision as of 17:12, 14 October 2008
Line 20:		Line 20:
	* May be better to store at 4 degrees C		* May be better to store at 4 degrees C
	* Dilute to appropriate concentration for use in biochemistry or molecular biology experiments. 20 mM or 50 mM Tris, pH 7.4 solutions are common.		* Dilute to appropriate concentration for use in biochemistry or molecular biology experiments. 20 mM or 50 mM Tris, pH 7.4 solutions are common.
		+
		+	== Stability ==
		+	* 18 months for Tris-HCl buffer according to this data sheet [http://www.labvision.com/pdf/uvdatasheet/AP-9005-XXX.pdf]
		+	* 2 yeasrs for Tris-EDTA according to this data sheet [http://www.labtech.co.uk/downloads/biothema/21-500.pdf]
		+

	[[Category:Material]] and [[Category:Buffers]]		[[Category:Material]] and [[Category:Buffers]]

2008-10-14T16:50:22Z

←Older revision		Revision as of 16:50, 14 October 2008
Line 1:		Line 1:
	[[Image:160px-Tris.png\|thumb\|Chemical structure of Tris - 2-Amino-2-hydroxymethyl-propane-1,3-diol]]		[[Image:160px-Tris.png\|thumb\|Chemical structure of Tris - 2-Amino-2-hydroxymethyl-propane-1,3-diol]]

-	[http://en.wikipedia.org/wiki/Tris Tris] is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C<sub>4</sub>H<sub>11</sub>NO<sub>3</sub>).	+	[http://en.wikipedia.org/wiki/Tris Tris] is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C<sub>4</sub>H<sub>11</sub>NO<sub>3</sub>, 2-Amino-2-hydroxymethyl-propane-1,3-diol).

	* The formula weight is 121.14		* The formula weight is 121.14

2008-10-14T16:49:48Z

figure: tris chemical structure

←Older revision		Revision as of 16:49, 14 October 2008
Line 1:		Line 1:
		+	[[Image:160px-Tris.png\|thumb\|Chemical structure of Tris - 2-Amino-2-hydroxymethyl-propane-1,3-diol]]
		+
	[http://en.wikipedia.org/wiki/Tris Tris] is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C<sub>4</sub>H<sub>11</sub>NO<sub>3</sub>).		[http://en.wikipedia.org/wiki/Tris Tris] is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C<sub>4</sub>H<sub>11</sub>NO<sub>3</sub>).

2008-03-11T21:36:42Z

added tags

←Older revision		Revision as of 21:36, 11 March 2008
Line 18:		Line 18:
	* May be better to store at 4 degrees C		* May be better to store at 4 degrees C
	* Dilute to appropriate concentration for use in biochemistry or molecular biology experiments. 20 mM or 50 mM Tris, pH 7.4 solutions are common.		* Dilute to appropriate concentration for use in biochemistry or molecular biology experiments. 20 mM or 50 mM Tris, pH 7.4 solutions are common.
		+
		+	[[Category:Material]] and [[Category:Buffers]]

2007-10-12T20:20:16Z

←Older revision		Revision as of 20:20, 12 October 2007
Line 1:		Line 1:
-	Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C<sub>4</sub>H<sub>11</sub>NO<sub>3</sub>).	+	[http://en.wikipedia.org/wiki/Tris Tris] is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C<sub>4</sub>H<sub>11</sub>NO<sub>3</sub>).

	* The formula weight is 121.14		* The formula weight is 121.14
Line 7:		Line 7:
	** Note that adding HCl during titration will affect the temperature of the solution		** Note that adding HCl during titration will affect the temperature of the solution
	* Dilution by a factor of 10 will reduce the pH by 0.1 pH units.		* Dilution by a factor of 10 will reduce the pH by 0.1 pH units.
		+
		+	== Preparing a 1M Tris, pH 7.4 solution, from [http://biologicalworld.com/trisbuffer.htm biologicalworld] ==
		+	* Weigh 121.1 g Tris in 700 ml double distilled water
		+	* Do not start with too much water; otherwise, the volume after pHing may be too great
		+	* Start stirring, and then check the pH
		+	* With a 3M HCl solution, start to pH the solution such that the pH reaches 7.4
		+	(Be careful not to inhale the HCl.)
		+	* Add with double distilled water up to 1L
		+	* Filter or autoclave if necessary
		+	* May be better to store at 4 degrees C
		+	* Dilute to appropriate concentration for use in biochemistry or molecular biology experiments. 20 mM or 50 mM Tris, pH 7.4 solutions are common.

2007-06-09T20:58:47Z

←Older revision		Revision as of 20:58, 9 June 2007
Line 4:		Line 4:
	* The pKa of Tris is 8.3		* The pKa of Tris is 8.3
	* dpK<sub>a</sub>/dT is -.028		* dpK<sub>a</sub>/dT is -.028
-	* A buffer at pH 6.8 at ~~25C~~ will have a pH of 6.46 at ~~37C~~	+	* A buffer at pH 6.8 at 25°C will have a pH of 6.46 at 37°C
	** Note that adding HCl during titration will affect the temperature of the solution		** Note that adding HCl during titration will affect the temperature of the solution
	* Dilution by a factor of 10 will reduce the pH by 0.1 pH units.		* Dilution by a factor of 10 will reduce the pH by 0.1 pH units.

2007-04-12T00:30:21Z

←Older revision		Revision as of 00:30, 12 April 2007
Line 1:		Line 1:
-	Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane.	+	Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C<sub>4</sub>H<sub>11</sub>NO<sub>3</sub>).

		+	* The formula weight is 121.14
	* The pKa of Tris is 8.3		* The pKa of Tris is 8.3
	* dpK<sub>a</sub>/dT is -.028		* dpK<sub>a</sub>/dT is -.028

←Older revision		Revision as of 17:17, 14 October 2008
Line 26:		Line 26:

	== See also ==		== See also ==
-	* [[TBS]] tris-buffered saline	+	* [[TBS]] - tris-buffered saline
-	* [[TBST]] tris-buffered saline with Tween	+	* [[TBST]] - tris-buffered saline with Tween

	[[Category:Material]]		[[Category:Material]]
	[[Category:Buffers]]		[[Category:Buffers]]

←Older revision		Revision as of 17:17, 14 October 2008
Line 25:		Line 25:
	* 2 years for Tris-EDTA according to this data sheet [http://www.labtech.co.uk/downloads/biothema/21-500.pdf]		* 2 years for Tris-EDTA according to this data sheet [http://www.labtech.co.uk/downloads/biothema/21-500.pdf]

		+	== See also ==
		+	* [[TBS]] tris-buffered saline
		+	* [[TBST]] tris-buffered saline with Tween

	[[Category:Material]]		[[Category:Material]]
	[[Category:Buffers]]		[[Category:Buffers]]