Tris

Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C₄H₁₁NO₃).

• The formula weight is 121.14
• The pKa of Tris is 8.3
• dpK_a/dT is -.028
• A buffer at pH 6.8 at 25°C will have a pH of 6.46 at 37°C
  • Note that adding HCl during titration will affect the temperature of the solution
• Dilution by a factor of 10 will reduce the pH by 0.1 pH units.

Preparing a 1M Tris, pH 7.4 solution, from biologicalworld

• Weigh 121.1 g Tris in 700 ml double distilled water
• Do not start with too much water; otherwise, the volume after pHing may be too great
• Start stirring, and then check the pH
• With a 3M HCl solution, start to pH the solution such that the pH reaches 7.4

(Be careful not to inhale the HCl.)

• Add with double distilled water up to 1L
• Filter or autoclave if necessary
• May be better to store at 4 degrees C
• Dilute to appropriate concentration for use in biochemistry or molecular biology experiments. 20 mM or 50 mM Tris, pH 7.4 solutions are common. and