Tris: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
Line 4: | Line 4: | ||
* dpK<sub>a</sub>/dT is -.028 | * dpK<sub>a</sub>/dT is -.028 | ||
* A buffer at pH 6.8 at 25C will have a pH of 6.46 at 37C | * A buffer at pH 6.8 at 25C will have a pH of 6.46 at 37C | ||
** Note that adding HCl during titration will affect the temperature of the solution | |||
* Dilution by a factor of 10 will reduce the pH by 0.1 pH units. | * Dilution by a factor of 10 will reduce the pH by 0.1 pH units. |
Revision as of 14:37, 12 September 2006
Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane.
- The pKa of Tris is 8.3
- dpKa/dT is -.028
- A buffer at pH 6.8 at 25C will have a pH of 6.46 at 37C
- Note that adding HCl during titration will affect the temperature of the solution
- Dilution by a factor of 10 will reduce the pH by 0.1 pH units.