Tris

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Line 4: Line 4:
* dpK<sub>a</sub>/dT is -.028
* dpK<sub>a</sub>/dT is -.028
* A buffer at pH 6.8 at 25C will have a pH of 6.46 at 37C
* A buffer at pH 6.8 at 25C will have a pH of 6.46 at 37C
 +
** Note that adding HCl during titration will affect the temperature of the solution
* Dilution by a factor of 10 will reduce the pH by 0.1 pH units.
* Dilution by a factor of 10 will reduce the pH by 0.1 pH units.

Revision as of 16:37, 12 September 2006

Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane.

  • The pKa of Tris is 8.3
  • dpKa/dT is -.028
  • A buffer at pH 6.8 at 25C will have a pH of 6.46 at 37C
    • Note that adding HCl during titration will affect the temperature of the solution
  • Dilution by a factor of 10 will reduce the pH by 0.1 pH units.
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