Tris
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| - | [http://en.wikipedia.org/wiki/Tris Tris] is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C<sub>4</sub>H<sub>11</sub>NO<sub>3</sub>). | + | [[Image:160px-Tris.png|thumb|Chemical structure of Tris - 2-Amino-2-hydroxymethyl-propane-1,3-diol]] |
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| + | [http://en.wikipedia.org/wiki/Tris Tris] is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C<sub>4</sub>H<sub>11</sub>NO<sub>3</sub>, 2-Amino-2-hydroxymethyl-propane-1,3-diol). | ||
* The formula weight is 121.14 | * The formula weight is 121.14 | ||
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* May be better to store at 4 degrees C | * May be better to store at 4 degrees C | ||
* Dilute to appropriate concentration for use in biochemistry or molecular biology experiments. 20 mM or 50 mM Tris, pH 7.4 solutions are common. | * Dilute to appropriate concentration for use in biochemistry or molecular biology experiments. 20 mM or 50 mM Tris, pH 7.4 solutions are common. | ||
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| + | == Stability == | ||
| + | * 18 months for Tris-HCl buffer according to this data sheet [http://www.labvision.com/pdf/uvdatasheet/AP-9005-XXX.pdf] | ||
| + | * 2 years for Tris-EDTA according to this data sheet [http://www.labtech.co.uk/downloads/biothema/21-500.pdf] | ||
| + | |||
| + | == See also == | ||
| + | * [[TBS]] - tris-buffered saline | ||
| + | * [[TBST]] - tris-buffered saline with Tween | ||
| + | |||
| + | [[Category:Material]] | ||
| + | [[Category:Buffers]] | ||
Current revision
Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C4H11NO3, 2-Amino-2-hydroxymethyl-propane-1,3-diol).
- The formula weight is 121.14
- The pKa of Tris is 8.3
- dpKa/dT is -.028
- A buffer at pH 6.8 at 25°C will have a pH of 6.46 at 37°C
- Note that adding HCl during titration will affect the temperature of the solution
- Dilution by a factor of 10 will reduce the pH by 0.1 pH units.
Preparing a 1M Tris, pH 7.4 solution, from biologicalworld
- Weigh 121.1 g Tris in 700 ml double distilled water
- Do not start with too much water; otherwise, the volume after pHing may be too great
- Start stirring, and then check the pH
- With a 3M HCl solution, start to pH the solution such that the pH reaches 7.4
(Be careful not to inhale the HCl.)
- Add with double distilled water up to 1L
- Filter or autoclave if necessary
- May be better to store at 4 degrees C
- Dilute to appropriate concentration for use in biochemistry or molecular biology experiments. 20 mM or 50 mM Tris, pH 7.4 solutions are common.
Stability
- 18 months for Tris-HCl buffer according to this data sheet [1]
- 2 years for Tris-EDTA according to this data sheet [2]
