Tris

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* The pKa of Tris is 8.3
* The pKa of Tris is 8.3
* dpK<sub>a</sub>/dT is -.028
* dpK<sub>a</sub>/dT is -.028
-
* A buffer at pH 6.8 at 25C will have a pH of 6.46 at 37C
+
* A buffer at pH 6.8 at 25&deg;C will have a pH of 6.46 at 37&deg;C
** Note that adding HCl during titration will affect the temperature of the solution
** Note that adding HCl during titration will affect the temperature of the solution
* Dilution by a factor of 10 will reduce the pH by 0.1 pH units.
* Dilution by a factor of 10 will reduce the pH by 0.1 pH units.

Revision as of 15:58, 9 June 2007

Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C4H11NO3).

  • The formula weight is 121.14
  • The pKa of Tris is 8.3
  • dpKa/dT is -.028
  • A buffer at pH 6.8 at 25°C will have a pH of 6.46 at 37°C
    • Note that adding HCl during titration will affect the temperature of the solution
  • Dilution by a factor of 10 will reduce the pH by 0.1 pH units.
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