Tris
From OpenWetWare
(Difference between revisions)
| Line 4: | Line 4: | ||
* The pKa of Tris is 8.3 | * The pKa of Tris is 8.3 | ||
* dpK<sub>a</sub>/dT is -.028 | * dpK<sub>a</sub>/dT is -.028 | ||
| - | * A buffer at pH 6.8 at | + | * A buffer at pH 6.8 at 25°C will have a pH of 6.46 at 37°C |
** Note that adding HCl during titration will affect the temperature of the solution | ** Note that adding HCl during titration will affect the temperature of the solution | ||
* Dilution by a factor of 10 will reduce the pH by 0.1 pH units. | * Dilution by a factor of 10 will reduce the pH by 0.1 pH units. | ||
Revision as of 16:58, 9 June 2007
Tris is a common buffering agent and is short for tris[hydroxymethyl]aminomethane (C4H11NO3).
- The formula weight is 121.14
- The pKa of Tris is 8.3
- dpKa/dT is -.028
- A buffer at pH 6.8 at 25°C will have a pH of 6.46 at 37°C
- Note that adding HCl during titration will affect the temperature of the solution
- Dilution by a factor of 10 will reduce the pH by 0.1 pH units.
