10 mM Tris–HCl + 0.05% Tween 20, pH 8.0 @ 25 °C
Purpose
- Tris buffers, with their pH adjusted by HCl, are often used to store DNA.
- Tween 20 is a non-ionic detergent that reduces adsorption of DNA to plastics and improves pipetting accuracy.
- This buffer is recommended (e.g. in the Kapa Library Quantification Kits) for diluting DNA before enzymatic reactions like qPCR, because unlike TE it does not contain EDTA, which could interfere with those reactions.
Recipe
- Final concentrations
| Tris base
|
10 mM
|
| Tween 20
|
0.05% (v/v)
|
| HCl
|
5.63 mM
|
- Preparing 50 mL of 1X working solution
| water
|
48.9685 mL
|
| Tris base, 1 M
|
0.5000 mL
|
| Tween 20, 10% (v/v)
|
0.2500 mL
|
| HCl, 1 N
|
0.2815 mL
|
- Preparing 50 mL of 10X concentrate
| water
|
39.685 mL
|
| Tris base, 1 M
|
5.000 mL
|
| Tween 20, 10% (v/v)
|
2.500 mL
|
| HCl, 1 N
|
2.815 mL
|
Notes
- HCl titration was calculated with the Python package ionize 0.8.0.
- pH is temperature-dependent and this dependency is especially strong for Tris, so pH indicators may show a noticeably higher pH than 8.0 at temperatures lower than 25 °C.
