Trimethoprim

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Contents

Mode of Action

Trimethoprim inhibits folate metabolism. Addition of folate to the medium can counteract trimethoprim.

Resistance Gene

Dihydrofolate Reductase (DHFR) is the gene counteracting trimethoprim.

Transposon Mutagenesis

Epicentre provides a Tn5 transposon system containing the DHFR gene for random knockouts of genes in bacteria.

Working concentrations and stock solutions

Working concentrations are typically 10 μg/ml. Trimethoprim is essentially insoluble, and very difficult to use as a stock solution. Addition of the powder is the easiest approach.

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