Trimethoprim - Revision history

Reshma P. Shetty at 17:49, 26 July 2007

2007-07-26T17:49:12Z

←Older revision		Revision as of 17:49, 26 July 2007
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	==Working concentrations and stock solutions==		==Working concentrations and stock solutions==
	Working concentrations are typically 10 μg/ml. Trimethoprim is essentially insoluble in water, but is soluble in DMSO (check the Merck index or Sigma Catalogue). Addition of the powder is also an option.		Working concentrations are typically 10 μg/ml. Trimethoprim is essentially insoluble in water, but is soluble in DMSO (check the Merck index or Sigma Catalogue). Addition of the powder is also an option.
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		+	[[Category:Material]] [[Category:Antibiotic]]

Tk at 20:39, 11 December 2006

2006-12-11T20:39:37Z

←Older revision		Revision as of 20:39, 11 December 2006
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	==Working concentrations and stock solutions==		==Working concentrations and stock solutions==
-	Working concentrations are typically 10 μg/ml. Trimethoprim is essentially insoluble in water, but is soluble in DMSO (check the ~~Merk~~ index or Sigma Catalogue). Addition of the powder is also an option.	+	Working concentrations are typically 10 μg/ml. Trimethoprim is essentially insoluble in water, but is soluble in DMSO (check the Merck index or Sigma Catalogue). Addition of the powder is also an option.

Qchristensen: Added content

2006-01-26T16:58:20Z

Added content

←Older revision		Revision as of 16:58, 26 January 2006
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	==Mode of Action==		==Mode of Action==
-	Trimethoprim inhibits folate ~~metabolism~~. Addition of folate to the medium can counteract trimethoprim.	+	Trimethoprim inhibits dihydrofolate reductase, most efficaciously effecting the bacterial enzyme. Bacteria are unable to uptake folate appreciably from rich medium and so must synthesize it themselves. Addition of folate to the medium can counteract trimethoprim, however.
		+
		+	Sulfonilamide effects another step in folate metabolism and so exibits a synergistic effect when used with trimethoprim.

	==Resistance Gene==		==Resistance Gene==
-	~~Dihydrofolate~~ Reductase (DHFR) is the gene counteracting trimethoprim.	+	An alternative dihydrofolate Reductase (DHFR) is the gene counteracting trimethoprim.

	==Transposon Mutagenesis==		==Transposon Mutagenesis==
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	==Working concentrations and stock solutions==		==Working concentrations and stock solutions==
-	Working concentrations are typically 10 μg/ml. Trimethoprim is essentially insoluble, ~~and very difficult to use as a stock solution~~. Addition of the powder is ~~the easiest approach~~.	+	Working concentrations are typically 10 μg/ml. Trimethoprim is essentially insoluble in water, but is soluble in DMSO (check the Merk index or Sigma Catalogue). Addition of the powder is also an option.

Tk at 19:50, 19 December 2005

2005-12-19T19:50:27Z

New page

==Mode of Action==
Trimethoprim inhibits folate metabolism. Addition of folate to the medium can counteract trimethoprim.

==Resistance Gene==
Dihydrofolate Reductase (DHFR) is the gene counteracting trimethoprim.

==Transposon Mutagenesis==
Epicentre provides a Tn5 transposon system containing the DHFR gene for random knockouts of genes in bacteria.

==Working concentrations and stock solutions==
Working concentrations are typically 10 μg/ml. Trimethoprim is essentially insoluble, and very difficult to use as a stock solution. Addition of the powder is the easiest approach.