Trimethoprim: Difference between revisions
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Revision as of 12:50, 19 December 2005
Mode of Action
Trimethoprim inhibits folate metabolism. Addition of folate to the medium can counteract trimethoprim.
Resistance Gene
Dihydrofolate Reductase (DHFR) is the gene counteracting trimethoprim.
Transposon Mutagenesis
Epicentre provides a Tn5 transposon system containing the DHFR gene for random knockouts of genes in bacteria.
Working concentrations and stock solutions
Working concentrations are typically 10 μg/ml. Trimethoprim is essentially insoluble, and very difficult to use as a stock solution. Addition of the powder is the easiest approach.
