Todd:TLC Stain Recipes: Difference between revisions
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Matthew Todd (talk | contribs) (New page: Stains are very useful for visualizing TLCs where certain compounds may be difficult to see under UV. Stains can also reveal different functional groups clearly. Keep all the stains togeth...) |
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* Vanillin: 3 g vanillin, 50 mL 95% ethanol, 15 mL 2 M H2SO4 | * Vanillin: 3 g vanillin, 50 mL 95% ethanol, 15 mL 2 M H2SO4 | ||
* Ceric Sulfate: 8 g Ce(SO4)2.2H2SO4, 100 mL 15% H2SO4 (aq) | * Ceric Sulfate: 8 g Ce(SO4)2.2H2SO4, 100 mL 15% H2SO4 (aq) | ||
* 2,4-DNP: 12 g 2,4- | * 2,4-DNP: 12 g 2,4-dinitrophenylhydrazine, 60 mL conc. H2SO4, 80 mL water, 200 mL 95% ethanol | ||
* Copper Sulfate: 3% cuSO4 (aq), 15% H3PO4 (aq) | * Copper Sulfate: 3% cuSO4 (aq), 15% H3PO4 (aq) | ||
* Ninhydrin: 0.3 g ninhydrin per 100 mL EtOH | * Ninhydrin: 0.3 g ninhydrin per 100 mL EtOH | ||
Data taken from [http://www.vanderbilt.edu/AnS/Chemistry/Rizzo/stuff/TLC.html this page]. Other sources for TLC stains...? | Data taken from [http://www.vanderbilt.edu/AnS/Chemistry/Rizzo/stuff/TLC.html this page]. Other sources for TLC stains...? | ||
Revision as of 03:29, 9 September 2010
Stains are very useful for visualizing TLCs where certain compounds may be difficult to see under UV. Stains can also reveal different functional groups clearly. Keep all the stains together in one hood/area, with a heat gun to hand. Always wipe the back of the plate with paper towel before heating to prevent excess stain dripping everywhere.
- Anisaldehyde: 9.2 mL p-anisaldehyde, 3.75 mL AcOH, 338 mL 95% ethanol, 12.5 mL conc. H2SO4
- Potassium Permanganate: 3 g KMnO4, 20 g K2CO3, 5 mL 5% NaOH (aq), 300 mL water
- PMA: 35 g phosphomolybdic acid, 1 pint 95% ethanol
- Ceric Ammonium Molybdate (Goofy's): 40 mL conc. H2SO4, 360 mL water, 10 g ammonium molybdate, 4 g ceric ammonium sulfate
- Vanillin: 3 g vanillin, 50 mL 95% ethanol, 15 mL 2 M H2SO4
- Ceric Sulfate: 8 g Ce(SO4)2.2H2SO4, 100 mL 15% H2SO4 (aq)
- 2,4-DNP: 12 g 2,4-dinitrophenylhydrazine, 60 mL conc. H2SO4, 80 mL water, 200 mL 95% ethanol
- Copper Sulfate: 3% cuSO4 (aq), 15% H3PO4 (aq)
- Ninhydrin: 0.3 g ninhydrin per 100 mL EtOH
Data taken from this page. Other sources for TLC stains...?
