Tk:wanted parts

• Expression biobrick plasmids
  • T7 promoter
  • T3
  • SP6
  • T7 RNA polymerase containing strains
  • also T3, SP6 What are the patent issues on these?

• xre protein, promoters (subtilis)

• antibiotic cassettes
  • Apramycin
  • Trimethoprim

• Transposase genes

• R6 origin plasmids

• chomosomal integration fragments
  • choice of locations on the chromosome

• cell killing enzymes
  • VSVG

• protein export tags

• protein cleavage domains

• protein purification tags, antibody epitopes
  • FLAG
    • DYKDDDDK, pmid 8770418
    • FLAG Epitope description
  • c-MYC
    • EQKLISEEDL
  • HA
    • YPYDVPDYA
  • 6HIS
    • HHHHHH
  • Strep
    • AWRHPQFGG
    • Ala-Trp-Arg-His-Pro-Gln-Phe-Gly-Gly
  • GST (n-terminal)
  • Chitin binding
  • BCCP biotin carboxyl carrier protein
    • PMID: 14736427
  • VSVG
  • Thrombin cleavage domain
    • LVPRGS (Leu-Val-Pro-Arg-Gly-Ser), factor Xa cleaved
  • Calmodulin tag
  • S-tag
  • GFP protein tags

• protein solubility
  • maltose binding protein
  • Thioredoxin

• protein splicing domains (inteins)

• surface display proteins
  • LPP/OmpA
  • Neisseria IgA1

• New fluorescent proteins
  • EBFP2 blue
  • new red monomers

• luminescent reporters
  • renilla
  • photuris
  • bacterial (Photorhabdus)
  • dual reporter renilla/photuris being done with John Cumbers (Brown)
  • dual reporter red/green beetle luciferase dual color

• enzymatic reporters
  • HRP protein
  • lacZ
  • Alkaline phosphatase

• Zinc finger domains

• Repair enzymes
  • UDG
  • mutS, mutC

• RNA sensor domains for all small molecules

• negative selection markers
  • sacB
  • pheS mutated

• toxic proteins
  • ccdB and similar

• Mesoplasma florum genes
  • recT, recE
  • GFP