Tk:video protocols: Difference between revisions
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** OD measurements | ** OD measurements | ||
** phases of growth | ** phases of growth | ||
* Receiving and sending strains and plasmids | |||
** recovering plasmids from spotted or dried DNA | |||
** growing stab cultures | |||
** preserving cultures in glycerol | |||
** preserving cultures with stabs | |||
** preserving cultures on plates | |||
** sending DNA | |||
** sending stabs | |||
** sending dry ice samples | |||
* Labels and lab notebooks | |||
** dated / initialed labels | |||
** lab notebooks | |||
| Line 39: | Line 56: | ||
** visualizing the bands | ** visualizing the bands | ||
** UV exposure issues | ** UV exposure issues | ||
* Cutting DNA from agarose gels | * Cutting DNA from agarose gels | ||
** UV exposure issues | ** UV exposure issues | ||
** weighing gel samples | ** weighing gel samples | ||
** | ** isolation of DNA from gel slices | ||
Latest revision as of 18:22, 10 November 2006
- Making plates / Media
- antibiotics
- weighing
- autoclaving
- pH
- water bath / prevention of stuff growing
- sterile technique
- material
- sterile filtration
- defined media
- Growing cultures
- spreaders / glass beads
- biosafety
- rotators
- aeration
- pH
- indicators
- temperature
- OD measurements
- phases of growth
- Receiving and sending strains and plasmids
- recovering plasmids from spotted or dried DNA
- growing stab cultures
- preserving cultures in glycerol
- preserving cultures with stabs
- preserving cultures on plates
- sending DNA
- sending stabs
- sending dry ice samples
- Labels and lab notebooks
- dated / initialed labels
- lab notebooks
- Agarose gels
- percentage choice
- buffer choice
- microwaving the gel
- adding dye
- pouring the gels
- bottle cleanup
- running the gel
- buffer choice
- adding dye to the buffer
- running time
- other kinds of agarose
- loading dye
- dna markers
- visualizing the bands
- UV exposure issues
- Cutting DNA from agarose gels
- UV exposure issues
- weighing gel samples
- isolation of DNA from gel slices
- PCR reactions
- primer design
- reaction components
- setting up the reaction
- choice of cycling conditions
- hot lids
