Tk:protein tags: Difference between revisions
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* Protein cleavage sites | * Protein cleavage sites [http://stevens.scripps.edu/webpage/htsb/cleavage.html] | ||
** Enterokinase | ** Enterokinase | ||
*** Asp-Asp-Asp-Asp-Lys↓ | *** Asp-Asp-Asp-Asp-Lys↓ |
Revision as of 11:02, 18 July 2008
- References
- PMID 12238768
- Current Protcols in Protein Science, section 9.9
- protein purification tags, antibody epitopes
- FLAG
- DYKDDDDK, pmid 8770418
- FLAG Epitope description
- c-MYC
- EQKLISEEDL
- HA
- YPYDVPDYA
- 6HIS
- HHHHHH
- Strep
- AWRHPQFGG
- Ala-Trp-Arg-His-Pro-Gln-Phe-Gly-Gly
- GST (n-terminal)
- Chitin binding
- BCCP biotin carboxyl carrier protein
- VSVG
- Thrombin cleavage domain
- LVPRGS (Leu-Val-Pro-Arg-Gly-Ser), factor Xa cleaved
- Calmodulin tag
- S-tag
- GFP protein tags
- FLAG
- protein solubility
- maltose binding protein
- Thioredoxin
- Protein cleavage sites [1]
- Enterokinase
- Asp-Asp-Asp-Asp-Lys↓
- some side cutting at basic residues
- Factor Xa
- Ile-Glu/Asp-Gly-Arg↓
- Thrombin
- Leu-Val-Pro-Arg↓Gly-Ser
- TEV protease
- Glu-Asn-Leu-Tyr-Phe-Gln↓Gly
- Pre-scission
- Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro
- Enterokinase