Tk:lab stuff: Difference between revisions
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(New page: ==At the bench== * mini centrifuge * fireboy * vortexer * Pipettor set * shelf rack for pipttors * Dumont Pipet * (some 8 well pipettors) * Dry block heater * pipet holder * bench light *...) |
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(33 intermediate revisions by the same user not shown) | |||
Line 6: | Line 6: | ||
* Pipettor set | * Pipettor set | ||
* shelf rack for pipttors | * shelf rack for pipttors | ||
* | * Drummond Pipettor | ||
* power strips | |||
* timer | |||
* (some 8 well pipettors) | * (some 8 well pipettors) | ||
* Dry block heater | * Dry block heater | ||
* gloves | |||
* Foam tube holders | |||
* water filled trays for melting reagents | |||
* ice buckets | |||
* pipet holder | * pipet holder | ||
* bench light | * bench light | ||
Line 21: | Line 27: | ||
* test tube rack (epoxy, also for 15 ml centrifuge tubes) | * test tube rack (epoxy, also for 15 ml centrifuge tubes) | ||
* rack for 50 ml centrifuge tubes | * rack for 50 ml centrifuge tubes | ||
* floaters | |||
* sharps disposal bin | * sharps disposal bin | ||
* containers for 2.0, 1.7, 0.5 thick, 0.5 thin tubes | * containers for 2.0, 1.7, 0.5 thick, 0.5 thin tubes | ||
* | * container for cryotubes, 0.5ml screw cap tubes | ||
* lab stool | |||
* lab coat | |||
* glass beads | |||
* toothpicks | |||
* spreader | |||
* Alcohol for spreader | |||
* parfilm | |||
* wash bottles (EtOH, water) | |||
* drawer | * drawer | ||
Line 37: | Line 51: | ||
** label maker | ** label maker | ||
** scotch tape | ** scotch tape | ||
** petri-stickers | |||
** stapler | |||
** scissors | |||
==autoclave== | |||
==autoclave | |||
* Yamato autoclave (or two) | * Yamato autoclave (or two) | ||
* autoclave tape | * autoclave tape | ||
* 1 liter beakers for water | * 1 liter beakers for water | ||
* drain pans | * drain pans | ||
* autoclave | * autoclave verification tests | ||
* hot gloves | * hot gloves | ||
* pouches for sterilization | |||
* Aluminum foil sheets | |||
==dishwasher== | ==dishwasher== | ||
* lab dish washer | * lab dish washer | ||
* alconox dishwasher soap | * alconox dishwasher soap | ||
* basket | * basket for caps | ||
* carts for bottles | |||
Line 69: | Line 87: | ||
==Scale== | ==Scale== | ||
* weigh boats | * weigh boats | ||
* weigh paper | |||
* dispsible spatulas | * dispsible spatulas | ||
* scale | * scale | ||
* brush | * brush | ||
* calibration weights | * calibration weights | ||
==pH meter== | ==pH meter== | ||
Line 83: | Line 101: | ||
* magnetic stir bars | * magnetic stir bars | ||
* magnetic stirrer | * magnetic stirrer | ||
* stir bar retrieval tool | |||
* pH paper | * pH paper | ||
==Sink== | ==Sink== | ||
Line 90: | Line 108: | ||
* Alcoscrub | * Alcoscrub | ||
* 70% Ethanol | * 70% Ethanol | ||
* Curel | |||
* Bleach | * Bleach | ||
* Liquid hand soap | * Liquid hand soap | ||
Line 100: | Line 119: | ||
* sponges | * sponges | ||
* mop | * mop | ||
* hazardous material gloves | |||
* large platic bins | |||
==Incubators== | ==Incubators== | ||
Line 136: | Line 157: | ||
* caps | * caps | ||
* lyophilizer freeze shelf / capping station | * lyophilizer freeze shelf / capping station | ||
* aluminum seal capper | |||
==Water purification sysem== | ==Water purification sysem== | ||
Line 144: | Line 165: | ||
==Supplies== | ==Supplies== | ||
* Petri dishes | * Petri dishes, 100mm, 60 mm | ||
* Pipet tips | * Pipet tips | ||
* Pipets | * Pipets 10, 25, 50 ml | ||
* Pipets 10 ml glass | |||
* Pasteur pipets | |||
* gloves | |||
* Microtiter plates | * Microtiter plates | ||
* Lids for microtiter plates | * Lids for microtiter plates | ||
* Tape for label makers | * Tape for label makers | ||
* hypodermics & needles | * hypodermics & needles 1, 3, 10, 20, 60 ml | ||
* 2.0, 1.7, 0.5 thick/thin, screw cap 2.0, 0.5, cryotubes | * 2.0, 1.7, 0.5 thick/thin, screw cap 2.0, 0.5, cryotubes | ||
* 2059 14 ml test tubes | * 2059 14 ml test tubes | ||
* 15 ml centrifuge tubes | * 15 ml centrifuge tubes | ||
* 50 ml centrifuge tubes | * 50 ml centrifuge tubes | ||
* foam insert freezer boxes | |||
* stainless steel freezer shelves | |||
* paper towels | * paper towels | ||
* sharps containers | * sharps containers | ||
Line 167: | Line 193: | ||
* lab notebooks | * lab notebooks | ||
* paper | * paper | ||
* Reagent reservoirs | |||
* gas cartridges for fireboys | |||
* clean air squirters | |||
* staples (large, small) | |||
* stapler removal tools | |||
* hole punch | |||
==Computers== | ==Computers== | ||
Line 177: | Line 208: | ||
* camera | * camera | ||
* software | * software | ||
* copier | |||
* binder | |||
==Electroporator== | |||
* zapper | |||
* cuvettes | |||
==Bead beater== | |||
* beater | |||
* Beads | |||
==Sonicator== | |||
==Protein Gels== | |||
* power supply | |||
* gel boxes | |||
* blotter | |||
* tilt table shaker | |||
* trays | |||
* Coumassie stain | |||
* Gel opener | |||
* drying frames | |||
==RNA bench== | |||
* distinguished pipettors | |||
* RNAse away | |||
* distinguished gel boxes | |||
* DEPC | |||
==Ice maker== | |||
* Ice buckets | |||
* scoops | |||
==Dry ice container== | |||
* with wheels | |||
==Freezers== | |||
* -80 upright with freezer drawers for boxes | |||
* -20 | |||
* chromatography style +4 | |||
* small under counter freezers and fridges | |||
==Centrifuges== | |||
* Large Eppendorf 5810R | |||
** fixed angle rotor with inserts for 15 and 50 ml tubes | |||
** swing rotor with plate, centrifuge tube for 15, 50, 300 ml tubes | |||
** all inserts required | |||
* Small benchtop 5817R with 30 position rotor | |||
==Microwave== | |||
* domestic microwave large enough for 1 liter bottles | |||
==Electrophoresis== | |||
* gel boxes | |||
* casting trays | |||
* gel trays | |||
* combs 8, 12, 15 | |||
* power supplies | |||
* Sybr Safe dye | |||
* DNA ladder (NEB 2 log ladder) | |||
* loading dye | |||
** ficol | |||
** orange G | |||
* paper towels | |||
* Clare dark reader | |||
* Razor blades | |||
* while light box | |||
* camera stand | |||
* saran wrap dispenser | |||
* TAE jugs | |||
* E Gel setup | |||
* supply of E-Gels | |||
* Gel imager | |||
** white trans/refl, UV 305, 365 trans | |||
** also western luminescent imager | |||
* hybridization oven | |||
* UV exposure system | |||
* bag sealer | |||
* 6 x 10 inch platic bags (sealable) | |||
==Glassware== | |||
* media bottles | |||
* Round 100 ml | |||
* Square 250, 500, 1000 ml | |||
* round 2 liter | |||
* extra caps | |||
** red hot caps | |||
** holed septum caps | |||
** extra seal rings (white, red) | |||
* Grey vented caps | |||
* Erlenmeyer flasks, 250, 500, 1000, 2000 ml with 45 ml lids | |||
* amber 100 ml round bottles | |||
* Graduates (plastic) 10, 25, 50, 100, 250, 1000, 4000 ml | |||
* separatory funnels | |||
* funnels | |||
* plastic bottles | |||
* Scoops | |||
* buchner funnels | |||
* corks | |||
* cork borer set | |||
* glass tubing (soft, pyrex) | |||
* capillary tubing | |||
* chemical bottles | |||
* volumetric flasks | |||
* sidearm flasks | |||
* glassware wash brushes | |||
* glassware cleaning beads | |||
* desiccator (w/ vacuum sidearm) | |||
* beakers | |||
** handles | |||
** glass | |||
** plastic, no handle | |||
* beaker tongs | |||
* hybridization bottles | |||
==Miscellaneous== | |||
* tubing | |||
* hazardous waste secondary containers | |||
* hand vacuum pumps | |||
* thermometers | |||
* replica plating tool | |||
* biohazard labels | |||
* chemical safety hazard labels | |||
==TLC system== | |||
* container | |||
* top | |||
* TLC membranes | |||
==Filters== | |||
* Whatman paper | |||
** sheets | |||
** circles | |||
* Microcon columns | |||
* Blotting paper | |||
* PVDF membranes | |||
* Nitrocellulose membranes | |||
* perforated sheets (hybridization) | |||
* Bottle top filters (0.2 u) | |||
* empty columns | |||
** spin columns | |||
** flow columns | |||
** luer valve | |||
==Large equipment== | |||
* sequencer | |||
* synthesizer | |||
* robot | |||
* mass spec | |||
* plate reader | |||
** luminescent | |||
** OD | |||
** fluorescent | |||
* quantitative PCR | |||
* PCR cycler | |||
* nanodrop (both spec and fluorescent) | |||
* CD | |||
* microcalorimetry | |||
* HPLC / FPLC | |||
* capillary electrophoresis | |||
* -80 freezer | |||
* -20 freezer | |||
* +4 chromatography fridge | |||
* Flaked ice maker | |||
* dishwasher | |||
* autoclaves (2) | |||
* DI water system | |||
* Eppendorf 5810R | |||
* Eppendorf 5817R | |||
* pH meter | |||
* freeze dryer | |||
* vacuum evaporator | |||
* New Brunswick shaker/incubator | |||
* backup power | |||
* microscope |
Latest revision as of 11:57, 6 December 2007
At the bench
- mini centrifuge
- fireboy
- vortexer
- Pipettor set
- shelf rack for pipttors
- Drummond Pipettor
- power strips
- timer
- (some 8 well pipettors)
- Dry block heater
- gloves
- Foam tube holders
- water filled trays for melting reagents
- ice buckets
- pipet holder
- bench light
- paper towels
- computer
- calculator
- lab notebook
- 80 hole eppendorf tube holders
- 96 hole PCCR tube holders
- 16 ml centrifuge tubes
- 60 ml centrifuge tubes
- test tube rack (epoxy, also for 15 ml centrifuge tubes)
- rack for 50 ml centrifuge tubes
- floaters
- sharps disposal bin
- containers for 2.0, 1.7, 0.5 thick, 0.5 thin tubes
- container for cryotubes, 0.5ml screw cap tubes
- lab stool
- lab coat
- glass beads
- toothpicks
- spreader
- Alcohol for spreader
- parfilm
- wash bottles (EtOH, water)
- drawer
- loop
- tweezers
- razor blades
- PCR 8 well strips
- caps for PCR strips
- pens
- lab markers
- lab tape
- label maker
- scotch tape
- petri-stickers
- stapler
- scissors
autoclave
- Yamato autoclave (or two)
- autoclave tape
- 1 liter beakers for water
- drain pans
- autoclave verification tests
- hot gloves
- pouches for sterilization
- Aluminum foil sheets
dishwasher
- lab dish washer
- alconox dishwasher soap
- basket for caps
- carts for bottles
fume hood
- vacuum system
- bottle top 0.2 u filters
- Pierce blow dry system
Plate pouring
- petri dishes
- lab tape
- water bath
- antibiotics
Scale
- weigh boats
- weigh paper
- dispsible spatulas
- scale
- brush
- calibration weights
pH meter
- Beckman pH meter
- Tris friendly electrode, 5mm
- calibration solutions
- pH storage solution
- pH filling solution
- magnetic stir bars
- magnetic stirrer
- stir bar retrieval tool
- pH paper
Sink
- paper towels
- Alcoscrub
- 70% Ethanol
- Curel
- Bleach
- Liquid hand soap
- Mat for bottom of sink
- water bath
- Evaporation beads for water bath
- Copper sulfate mold prevention for water bath
- Acid Base Biohazard spill kits
- buckets
- sponges
- mop
- hazardous material gloves
- large platic bins
Incubators
- New Brunswick shaker
- various size holders
- screws, screwdrivers
- rack for tubes
- VWR 37 and 30 incubators
- rotators
- shakers
- small shaking incubator (Eppendorf)
PCR machines
- Opticon or equivalent
- dual block 0.5 ml alpha unit
- 8 well strips and caps
- some plates
Spectrophotometer
- Nanodrop
- kimwipes
- PC for software
Plate Reader
Vacuum evaporator
Freeze Dryer
- torch
- serum bottles
- caps
- lyophilizer freeze shelf / capping station
- aluminum seal capper
Water purification sysem
Supplies
- Petri dishes, 100mm, 60 mm
- Pipet tips
- Pipets 10, 25, 50 ml
- Pipets 10 ml glass
- Pasteur pipets
- gloves
- Microtiter plates
- Lids for microtiter plates
- Tape for label makers
- hypodermics & needles 1, 3, 10, 20, 60 ml
- 2.0, 1.7, 0.5 thick/thin, screw cap 2.0, 0.5, cryotubes
- 2059 14 ml test tubes
- 15 ml centrifuge tubes
- 50 ml centrifuge tubes
- foam insert freezer boxes
- stainless steel freezer shelves
- paper towels
- sharps containers
- autoclave bags
- biohazard bins
- hazardous waste labels
- lab tape
- autoclave tape
- scotch tape
- lab markers
- pens
- lab notebooks
- paper
- Reagent reservoirs
- gas cartridges for fireboys
- clean air squirters
- staples (large, small)
- stapler removal tools
- hole punch
Computers
- fax machine
- printer (color)
- internet access
- scanner
- video recorder
- camera
- software
- copier
- binder
Electroporator
- zapper
- cuvettes
Bead beater
- beater
- Beads
Sonicator
Protein Gels
- power supply
- gel boxes
- blotter
- tilt table shaker
- trays
- Coumassie stain
- Gel opener
- drying frames
RNA bench
- distinguished pipettors
- RNAse away
- distinguished gel boxes
- DEPC
Ice maker
- Ice buckets
- scoops
Dry ice container
- with wheels
Freezers
- -80 upright with freezer drawers for boxes
- -20
- chromatography style +4
- small under counter freezers and fridges
Centrifuges
- Large Eppendorf 5810R
- fixed angle rotor with inserts for 15 and 50 ml tubes
- swing rotor with plate, centrifuge tube for 15, 50, 300 ml tubes
- all inserts required
- Small benchtop 5817R with 30 position rotor
Microwave
- domestic microwave large enough for 1 liter bottles
Electrophoresis
- gel boxes
- casting trays
- gel trays
- combs 8, 12, 15
- power supplies
- Sybr Safe dye
- DNA ladder (NEB 2 log ladder)
- loading dye
- ficol
- orange G
- paper towels
- Clare dark reader
- Razor blades
- while light box
- camera stand
- saran wrap dispenser
- TAE jugs
- E Gel setup
- supply of E-Gels
- Gel imager
- white trans/refl, UV 305, 365 trans
- also western luminescent imager
- hybridization oven
- UV exposure system
- bag sealer
- 6 x 10 inch platic bags (sealable)
Glassware
- media bottles
- Round 100 ml
- Square 250, 500, 1000 ml
- round 2 liter
- extra caps
- red hot caps
- holed septum caps
- extra seal rings (white, red)
- Grey vented caps
- Erlenmeyer flasks, 250, 500, 1000, 2000 ml with 45 ml lids
- amber 100 ml round bottles
- Graduates (plastic) 10, 25, 50, 100, 250, 1000, 4000 ml
- separatory funnels
- funnels
- plastic bottles
- Scoops
- buchner funnels
- corks
- cork borer set
- glass tubing (soft, pyrex)
- capillary tubing
- chemical bottles
- volumetric flasks
- sidearm flasks
- glassware wash brushes
- glassware cleaning beads
- desiccator (w/ vacuum sidearm)
- beakers
- handles
- glass
- plastic, no handle
- beaker tongs
- hybridization bottles
Miscellaneous
- tubing
- hazardous waste secondary containers
- hand vacuum pumps
- thermometers
- replica plating tool
- biohazard labels
- chemical safety hazard labels
TLC system
- container
- top
- TLC membranes
Filters
- Whatman paper
- sheets
- circles
- Microcon columns
- Blotting paper
- PVDF membranes
- Nitrocellulose membranes
- perforated sheets (hybridization)
- Bottle top filters (0.2 u)
- empty columns
- spin columns
- flow columns
- luer valve
Large equipment
- sequencer
- synthesizer
- robot
- mass spec
- plate reader
- luminescent
- OD
- fluorescent
- quantitative PCR
- PCR cycler
- nanodrop (both spec and fluorescent)
- CD
- microcalorimetry
- HPLC / FPLC
- capillary electrophoresis
- -80 freezer
- -20 freezer
- +4 chromatography fridge
- Flaked ice maker
- dishwasher
- autoclaves (2)
- DI water system
- Eppendorf 5810R
- Eppendorf 5817R
- pH meter
- freeze dryer
- vacuum evaporator
- New Brunswick shaker/incubator
- backup power
- microscope