Tk:lab stuff: Difference between revisions

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Line 6: Line 6:
* Pipettor set
* Pipettor set
* shelf rack for pipttors
* shelf rack for pipttors
* Dumont Pipet
* Drummond Pipettor
* power strips
* timer
* (some 8 well pipettors)
* (some 8 well pipettors)
* Dry block heater
* Dry block heater
* gloves
* Foam tube holders
* Foam tube holders
* water filled trays for melting reagents
* water filled trays for melting reagents
Line 24: Line 27:
* test tube rack (epoxy, also for 15 ml centrifuge tubes)
* test tube rack (epoxy, also for 15 ml centrifuge tubes)
* rack for 50 ml centrifuge tubes
* rack for 50 ml centrifuge tubes
* floaters
* sharps disposal bin
* sharps disposal bin
* containers for 2.0, 1.7, 0.5 thick, 0.5 thin tubes
* containers for 2.0, 1.7, 0.5 thick, 0.5 thin tubes
* containser for cryotubes, 0.5ml screw cap tubes
* container for cryotubes, 0.5ml screw cap tubes
 
* lab stool
* lab coat
* glass beads
* toothpicks
* spreader
* Alcohol for spreader
* parfilm
* wash bottles (EtOH, water)


* drawer
* drawer
Line 41: Line 52:
** scotch tape
** scotch tape
** petri-stickers
** petri-stickers
** stapler
** scissors


==autoclave--
==autoclave==
* Yamato autoclave (or two)
* Yamato autoclave (or two)
* autoclave tape
* autoclave tape
* 1 liter beakers for water
* 1 liter beakers for water
* drain pans
* drain pans
* autoclave verrification tests
* autoclave verification tests
* hot gloves
* hot gloves
 
* pouches for sterilization
* Aluminum foil sheets


==dishwasher==
==dishwasher==
Line 73: Line 87:
==Scale==
==Scale==
* weigh boats
* weigh boats
* weigh paper
* dispsible spatulas
* dispsible spatulas
* scale
* scale
* brush
* brush
* calibration weights
* calibration weights


==pH meter==
==pH meter==
Line 87: Line 101:
* magnetic stir bars
* magnetic stir bars
* magnetic stirrer
* magnetic stirrer
* stir bar retrieval tool
* pH paper
* pH paper


==Sink==
==Sink==
Line 94: Line 108:
* Alcoscrub
* Alcoscrub
* 70% Ethanol
* 70% Ethanol
* Curel
* Bleach
* Bleach
* Liquid hand soap
* Liquid hand soap
Line 104: Line 119:
* sponges
* sponges
* mop
* mop
* hazardous material gloves
* large platic bins


==Incubators==
==Incubators==
Line 140: Line 157:
* caps
* caps
* lyophilizer freeze shelf / capping station
* lyophilizer freeze shelf / capping station
 
* aluminum seal capper


==Water purification sysem==
==Water purification sysem==
Line 153: Line 170:
* Pipets 10 ml glass
* Pipets 10 ml glass
* Pasteur pipets
* Pasteur pipets
* gloves
* Microtiter plates
* Microtiter plates
* Lids for microtiter plates
* Lids for microtiter plates
Line 176: Line 194:
* paper
* paper
* Reagent reservoirs
* Reagent reservoirs
* gas cartridges for fireboys
* clean air squirters
* staples (large, small)
* stapler removal tools
* hole punch


==Computers==
==Computers==
Line 185: Line 208:
* camera
* camera
* software
* software
 
* copier
* binder


==Electroporator==
==Electroporator==
Line 208: Line 232:
* Coumassie stain
* Coumassie stain
* Gel opener
* Gel opener
 
* drying frames


==RNA bench==
==RNA bench==
Line 243: Line 267:
==Microwave==
==Microwave==
* domestic microwave large enough for 1 liter bottles
* domestic microwave large enough for 1 liter bottles
==Electrophoresis==
* gel boxes
* casting trays
* gel trays
* combs 8, 12, 15
* power supplies
* Sybr Safe dye
* DNA ladder (NEB 2 log ladder)
* loading dye
** ficol
** orange G
* paper towels
* Clare dark reader
* Razor blades
* while light box
* camera stand
* saran wrap dispenser
* TAE jugs
* E Gel setup
* supply of E-Gels
* Gel imager
** white trans/refl, UV 305, 365 trans
** also western luminescent imager
* hybridization oven
* UV exposure system
* bag sealer
* 6 x 10 inch platic bags (sealable)
==Glassware==
* media bottles
* Round 100 ml
* Square 250, 500, 1000 ml
* round 2 liter
* extra caps
** red hot caps
** holed septum caps
** extra seal rings (white, red)
* Grey vented caps
* Erlenmeyer flasks, 250, 500, 1000, 2000 ml with 45 ml lids
* amber 100 ml round bottles
* Graduates (plastic) 10, 25, 50, 100, 250, 1000, 4000 ml
* separatory funnels
* funnels
* plastic bottles
* Scoops
* buchner funnels
* corks
* cork borer set
* glass tubing (soft, pyrex)
* capillary tubing
* chemical bottles
* volumetric flasks
* sidearm flasks
* glassware wash brushes
* glassware cleaning beads
* desiccator (w/ vacuum sidearm)
* beakers
** handles
** glass
** plastic, no handle
* beaker tongs
* hybridization bottles
==Miscellaneous==
* tubing
* hazardous waste secondary containers
* hand vacuum pumps
* thermometers
* replica plating tool
* biohazard labels
* chemical safety hazard labels
==TLC system==
* container
* top
* TLC membranes
==Filters==
* Whatman paper
** sheets
** circles
* Microcon columns
* Blotting paper
* PVDF membranes
* Nitrocellulose membranes
* perforated sheets (hybridization)
* Bottle top filters (0.2 u)
* empty columns
** spin columns
** flow columns
** luer valve
==Large equipment==
* sequencer
* synthesizer
* robot
* mass spec
* plate reader
** luminescent
** OD
** fluorescent
* quantitative PCR
* PCR cycler
* nanodrop (both spec and fluorescent)
* CD
* microcalorimetry
* HPLC / FPLC
* capillary electrophoresis
* -80 freezer
* -20 freezer
* +4 chromatography fridge
* Flaked ice maker
* dishwasher
* autoclaves (2)
* DI water system
* Eppendorf 5810R
* Eppendorf 5817R
* pH meter
* freeze dryer
* vacuum evaporator
* New Brunswick shaker/incubator
* backup power
* microscope

Latest revision as of 11:57, 6 December 2007

At the bench

  • mini centrifuge
  • fireboy
  • vortexer
  • Pipettor set
  • shelf rack for pipttors
  • Drummond Pipettor
  • power strips
  • timer
  • (some 8 well pipettors)
  • Dry block heater
  • gloves
  • Foam tube holders
  • water filled trays for melting reagents
  • ice buckets
  • pipet holder
  • bench light
  • paper towels
  • computer
  • calculator
  • lab notebook
  • 80 hole eppendorf tube holders
  • 96 hole PCCR tube holders
  • 16 ml centrifuge tubes
  • 60 ml centrifuge tubes
  • test tube rack (epoxy, also for 15 ml centrifuge tubes)
  • rack for 50 ml centrifuge tubes
  • floaters
  • sharps disposal bin
  • containers for 2.0, 1.7, 0.5 thick, 0.5 thin tubes
  • container for cryotubes, 0.5ml screw cap tubes
  • lab stool
  • lab coat
  • glass beads
  • toothpicks
  • spreader
  • Alcohol for spreader
  • parfilm
  • wash bottles (EtOH, water)
  • drawer
    • loop
    • tweezers
    • razor blades
    • PCR 8 well strips
    • caps for PCR strips
    • pens
    • lab markers
    • lab tape
    • label maker
    • scotch tape
    • petri-stickers
    • stapler
    • scissors

autoclave

  • Yamato autoclave (or two)
  • autoclave tape
  • 1 liter beakers for water
  • drain pans
  • autoclave verification tests
  • hot gloves
  • pouches for sterilization
  • Aluminum foil sheets

dishwasher

  • lab dish washer
  • alconox dishwasher soap
  • basket for caps
  • carts for bottles


fume hood

  • vacuum system
  • bottle top 0.2 u filters
  • Pierce blow dry system


Plate pouring

  • petri dishes
  • lab tape
  • water bath
  • antibiotics


Scale

  • weigh boats
  • weigh paper
  • dispsible spatulas
  • scale
  • brush
  • calibration weights

pH meter

  • Beckman pH meter
  • Tris friendly electrode, 5mm
  • calibration solutions
  • pH storage solution
  • pH filling solution
  • magnetic stir bars
  • magnetic stirrer
  • stir bar retrieval tool
  • pH paper

Sink

  • paper towels
  • Alcoscrub
  • 70% Ethanol
  • Curel
  • Bleach
  • Liquid hand soap
  • Mat for bottom of sink
  • water bath
  • Evaporation beads for water bath
  • Copper sulfate mold prevention for water bath
  • Acid Base Biohazard spill kits
  • buckets
  • sponges
  • mop
  • hazardous material gloves
  • large platic bins

Incubators

  • New Brunswick shaker
    • various size holders
    • screws, screwdrivers
    • rack for tubes
  • VWR 37 and 30 incubators
  • rotators
  • shakers
  • small shaking incubator (Eppendorf)


PCR machines

  • Opticon or equivalent
  • dual block 0.5 ml alpha unit
  • 8 well strips and caps
  • some plates


Spectrophotometer

  • Nanodrop
  • kimwipes
  • PC for software


Plate Reader

Vacuum evaporator

Freeze Dryer

  • torch
  • serum bottles
  • caps
  • lyophilizer freeze shelf / capping station
  • aluminum seal capper

Water purification sysem

Supplies

  • Petri dishes, 100mm, 60 mm
  • Pipet tips
  • Pipets 10, 25, 50 ml
  • Pipets 10 ml glass
  • Pasteur pipets
  • gloves
  • Microtiter plates
  • Lids for microtiter plates
  • Tape for label makers
  • hypodermics & needles 1, 3, 10, 20, 60 ml
  • 2.0, 1.7, 0.5 thick/thin, screw cap 2.0, 0.5, cryotubes
  • 2059 14 ml test tubes
  • 15 ml centrifuge tubes
  • 50 ml centrifuge tubes
  • foam insert freezer boxes
  • stainless steel freezer shelves
  • paper towels
  • sharps containers
  • autoclave bags
  • biohazard bins
  • hazardous waste labels
  • lab tape
  • autoclave tape
  • scotch tape
  • lab markers
  • pens
  • lab notebooks
  • paper
  • Reagent reservoirs
  • gas cartridges for fireboys
  • clean air squirters
  • staples (large, small)
  • stapler removal tools
  • hole punch

Computers

  • fax machine
  • printer (color)
  • internet access
  • scanner
  • video recorder
  • camera
  • software
  • copier
  • binder

Electroporator

  • zapper
  • cuvettes


Bead beater

  • beater
  • Beads


Sonicator

Protein Gels

  • power supply
  • gel boxes
  • blotter
  • tilt table shaker
  • trays
  • Coumassie stain
  • Gel opener
  • drying frames

RNA bench

  • distinguished pipettors
  • RNAse away
  • distinguished gel boxes
  • DEPC


Ice maker

  • Ice buckets
  • scoops


Dry ice container

  • with wheels


Freezers

  • -80 upright with freezer drawers for boxes
  • -20
  • chromatography style +4
  • small under counter freezers and fridges


Centrifuges

  • Large Eppendorf 5810R
    • fixed angle rotor with inserts for 15 and 50 ml tubes
    • swing rotor with plate, centrifuge tube for 15, 50, 300 ml tubes
    • all inserts required
  • Small benchtop 5817R with 30 position rotor


Microwave

  • domestic microwave large enough for 1 liter bottles


Electrophoresis

  • gel boxes
  • casting trays
  • gel trays
  • combs 8, 12, 15
  • power supplies
  • Sybr Safe dye
  • DNA ladder (NEB 2 log ladder)
  • loading dye
    • ficol
    • orange G
  • paper towels
  • Clare dark reader
  • Razor blades
  • while light box
  • camera stand
  • saran wrap dispenser
  • TAE jugs
  • E Gel setup
  • supply of E-Gels
  • Gel imager
    • white trans/refl, UV 305, 365 trans
    • also western luminescent imager
  • hybridization oven
  • UV exposure system
  • bag sealer
  • 6 x 10 inch platic bags (sealable)

Glassware

  • media bottles
  • Round 100 ml
  • Square 250, 500, 1000 ml
  • round 2 liter
  • extra caps
    • red hot caps
    • holed septum caps
    • extra seal rings (white, red)
  • Grey vented caps
  • Erlenmeyer flasks, 250, 500, 1000, 2000 ml with 45 ml lids
  • amber 100 ml round bottles
  • Graduates (plastic) 10, 25, 50, 100, 250, 1000, 4000 ml
  • separatory funnels
  • funnels
  • plastic bottles
  • Scoops
  • buchner funnels
  • corks
  • cork borer set
  • glass tubing (soft, pyrex)
  • capillary tubing
  • chemical bottles
  • volumetric flasks
  • sidearm flasks
  • glassware wash brushes
  • glassware cleaning beads
  • desiccator (w/ vacuum sidearm)
  • beakers
    • handles
    • glass
    • plastic, no handle
  • beaker tongs
  • hybridization bottles


Miscellaneous

  • tubing
  • hazardous waste secondary containers
  • hand vacuum pumps
  • thermometers
  • replica plating tool
  • biohazard labels
  • chemical safety hazard labels

TLC system

  • container
  • top
  • TLC membranes

Filters

  • Whatman paper
    • sheets
    • circles
  • Microcon columns
  • Blotting paper
  • PVDF membranes
  • Nitrocellulose membranes
  • perforated sheets (hybridization)
  • Bottle top filters (0.2 u)
  • empty columns
    • spin columns
    • flow columns
    • luer valve

Large equipment

  • sequencer
  • synthesizer
  • robot
  • mass spec
  • plate reader
    • luminescent
    • OD
    • fluorescent
  • quantitative PCR
  • PCR cycler
  • nanodrop (both spec and fluorescent)
  • CD
  • microcalorimetry
  • HPLC / FPLC
  • capillary electrophoresis
  • -80 freezer
  • -20 freezer
  • +4 chromatography fridge
  • Flaked ice maker
  • dishwasher
  • autoclaves (2)
  • DI water system
  • Eppendorf 5810R
  • Eppendorf 5817R
  • pH meter
  • freeze dryer
  • vacuum evaporator
  • New Brunswick shaker/incubator
  • backup power
  • microscope