Tk:E. coli cut sites
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- CTAG sequences in the E. coli genome are rare.
- SpeI cut sites in the E.coli genome: 74
- XbaI cut sites in the E. coli genome: 40
- PstI cut sites in a 300Kbp region of the E. coli genome: 79
- Average length of PstI fragment: 300K/79 = 3800
- We would expect the average length of a double cut XbaI / PstI fragment to be about 1900 bp, and there to be about 40 different ones.
- Likely there are a few with order of 100 bp length
- EcoRI cut sites in a 300Kbp region of the E. coli genome: 42, but 18 are methylation inhibited. 24 are left actually cut.
- Average length of EcoRI fragment: 300K/24 = 12500
- We would expect the average length of the double cut EcoRI / SpeI to be about 6250 bp, and there to be about 79 of them.
- Likely there are a few with order of 80 bp length
- 1-300KB
- 11828 SpeI ACTAGT
- 25681 XbaI TCTAGA
- 84649 SpeI ACTAGT
- 131428 XbaI TCTAGA
- 237191 XbaI TCTAGA
- 270729 XbaI TCTAGA
- 310919 SpeI ACTAGT
- 380545 SpeI ACTAGT
- 380557 SpeI ACTAGT
- 405554 SpeI ACTAGT
- 445916 SpeI ACTAGT
- 570189 SpeI ACTAGT
- 594695 XbaI TCTAGA
- 849339 XbaI TCTAGA
- 889063 SpeI ACTAGT
- 889173 SpeI ACTAGT
- 921898 XbaI TCTAGA
- 968572 SpeI ACTAGT
- 1098843 SpeI ACTAGT
- 1184031 XbaI TCTAGA
- 1195749 SpeI ACTAGT
- 1196554 XbaI TCTAGA
- 1196673 XbaI TCTAGA
- 1198740 XbaI TCTAGA
- 1214824 SpeI ACTAGT
- 1219071 SpeI ACTAGT
- 1321138 SpeI ACTAGT
- 1321146 SpeI ACTAGT
- 1343440 # SpeI ACTAGT
- 1467188 SpeI ACTAGT
- 1467200 SpeI ACTAGT
- 1468285 XbaI TCTAGA
- 1494788 XbaI TCTAGA
- 1522924 SpeI ACTAGT
- 1566793 SpeI ACTAGT
- 1686509 SpeI ACTAGT
- 1695103 SpeI ACTAGT
- 1710491 SpeI ACTAGT
- 1743206 XbaI TCTAGA
- 1745491 SpeI ACTAGT
- 1755429 XbaI TCTAGA
- 1786307 SpeI ACTAGT
- 1970292 XbaI TCTAGA
- 2001080 SpeI ACTAGT
- 2009992 XbaI TCTAGA
- 2068219 SpeI ACTAGT
- 2068231 SpeI ACTAGT
- 2088067 SpeI ACTAGT
- 2096253 SpeI ACTAGT
