http://openwetware.org/index.php?title=Thermostable_DNA_ligase&feed=atom&action=history 2013-05-19T04:47:15Z Revision history for this page on the wiki MediaWiki 1.13.2 http://openwetware.org/index.php?title=Thermostable_DNA_ligase&diff=196473&oldid=prev 2008-04-14T08:43:55Z

<p>New page: ==Purpose== *Thermostable DNA ligases can be added to a PCR reaction to ligate extended oligos. This is used for some mutagenesis methods. ==Procurement== *Examples for thermostable DNA l...</p> <p><b>New page</b></p><div>==Purpose==<br /> *Thermostable DNA ligases can be added to a PCR reaction to ligate extended oligos. This is used for some mutagenesis methods.<br /> <br /> ==Procurement==<br /> *Examples for thermostable DNA ligases are Taq ligase [http://www.neb.com/nebecomm/products/productM0208.asp NEB] and the Ampligase [http://www.epibio.com/item.asp?ID=265 Epicentre].<br /> <br /> ==Use==<br /> *If you want to ligate extended oligos in a PCR reaction it is important to have them phosphorilated at the 5'-end. Oligos will only be ligated when they are assembled on a compementary strand.<br /> <br /> <br /> [[Category:Material]] [[Category:Enzyme]] [[Category:Ligase]] [[Category:DNA]]</div>

Torsten Waldminghaus