The BioBricks Foundation:Standards/Technical/BBF WetLab Challenges

BBF WetLab Challenges

Vincent 05:05, 11 February 2008 (CST): I tried to summarize the ongoing discussion. Feel free to edit as you like.

Suggested Mission Statement

To bring the BBF community together ,on a regular basis, to support and challenge the establishment of wet-lab related standards in the area of biological engineering.

Benefits

• Build critical mass to generate valuable experimental data
• Test reliability and reproducibility of proposed Standards
• Foster BBF community

Targeted Audience

• BBF enthusiasts
• iGEM students

Possible topics

• Standard Calibration/Measurement procedures:
  • Endy:Measuring_PoPS_on_a_plate_reader
  • Standardized_GFP_quantification
• Characterisation of basic parts: (Promoters, terminators, RBS, Inverters)
  • Registry/Measurement_kit
  • Endy:Screening_plasmid
  • pLac / pBad promoters
• Distributed 'Design of Experiment' on a specific device/system (for sensitivity/robustness analysis)

Suggested workflow

1. Define a list of parts ,or protocols, to be challenged/characterized --online--
2. BFF community vote for the top priority (doodle type) --online--
3. Define a common protocol to follow on the day --online--
4. Define how the data are going to be stored/shared for future analysis --online--
5. Organize wet lab session --face to face--
6. Collect data and share them (take pictures and video) --face to face--
7. Analysis and discussion of the outcomes --online--

1. a new cycle starts ...

Practical Issues

• 1-2 days activities (week-end type of events), it is a constrain for some experimental work.
• Every 4-6 months
• Centralized versus Decentratized wet lab organization
  • Vincent 05:05, 11 February 2008 (CST): I would prefer centralized as it helps to build community spirit, at least regional-type of gathering.
• Storing and Sharing data generated: Where ? How ? Curation ?
• find a catchy name for the event

Resources

• Discussion started on the Technical Standard Mailing List, Feb 2008.
• Technical Resources
• Similar type of event in other areas:
  • google summer code
  • Make fair