Tessa A. Morris Week 10
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Article
Transcriptional Regulatory Networks in Saccharomyces cerevisiae
10 Biological Terms
- Nucleate
- Motifs
- Genome-wide location analysis
- myc epitope tag
- Immunoblot analysis
- Peptone
- Dextrose
- Immunoprecipitated
- Thiamine
- Fhl1 function
- Intergenic
Outline
Abstract
- It was determined how most transcriptional regulators encoded in Saccharomyces cerevisiae associate with genes across the genome in
living cells, which can be used to describe potential pathways yeast cells can use to regulate global gene expression programs
- In this experiment, the scientists used this information to identify network motifs (simplest units of network architecture) and show that an automated process can use motifs to assemble a transcription regulatory network structure
- Results: eukaryotic cellular functions are highly connected through networks of transcriptional regulators that regulate other transcriptional regulators
Introduction
Questions
- What is the main result presented in this paper?
- What is the importance or significance of this work?
- Briefly describe their methods, including the following information. A flow chart may be helpful here.
- How did they treat the cells (what experiment were they doing?)
- What strain(s) of yeast did they use? Was the strain haploid or diploid?
- What media did they grow them in? Under what conditions and temperatures?
- What controls did they use?
- How many replicates did they perform per condition?
- What mathematical/statistical method did they use to analyze the data?
- What transcription factors did they talk about?
- Briefly state the result shown in each of the figures and tables.
Presentation
Partners: Kristen M. Horstmann and Lucia I. Ramirez
User Page: Tessa A. Morris
Course Page: Biomathematical Modeling
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