Terrific Broth

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Summary

Terrific broth (TB, not to be confused with T Broth)

Ingredients

  1. 12 g Bacto tryptone
  2. 24 g Bacto yeast extract
  3. 4 mL Glycerol
  4. 100 mL 0.17M KH2PO4 and 0.72M K2HPO4, sterile, prepared separately

Protocol

  1. Mix tryptone, yeast extract and glycerol, and add distilled water up to 900mL
  2. Pour into 2 L flask (or greater)
  3. Autoclave (liquid cycle)
  4. Allow liquid to cool to less than 60 degrees Centrigrade
  5. Add 100 mL potassium phosphate solution
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