Terrific Broth - Revision history

Torsten Waldminghaus: added tags

Torsten Waldminghaus — Tue, 11 Mar 2008 21:13:09 GMT

added tags

←Older revision		Revision as of 21:13, 11 March 2008
Line 13:		Line 13:
	#Allow liquid to cool to less than 60 degrees Centrigrade		#Allow liquid to cool to less than 60 degrees Centrigrade
	#Add 100 mL [[potassium phosphate solution]]		#Add 100 mL [[potassium phosphate solution]]
		+
		+	[[Category:Material]] [[Category:Standard Media]]

CnorthMSU: Rephrasing of potassium phosphate solution for better clarity

CnorthMSU — Tue, 19 Dec 2006 15:15:10 GMT

Rephrasing of potassium phosphate solution for better clarity

←Older revision		Revision as of 15:15, 19 December 2006
Line 5:		Line 5:
	#24 g Bacto yeast extract		#24 g Bacto yeast extract
	#4 mL Glycerol		#4 mL Glycerol
-	#100 mL 0.17M KH2PO4 and 0.72M K2HPO4, sterile, prepared separately	+	#100 mL 0.17M KH2PO4 and 0.72M K2HPO4, sterile, to be prepared separately from the tryptone, yeast extract, and glycerol solution.

	==Protocol==		==Protocol==

CnorthMSU at 17:08, 14 December 2006

CnorthMSU — Thu, 14 Dec 2006 17:08:07 GMT

←Older revision		Revision as of 17:08, 14 December 2006
Line 11:		Line 11:
	#Pour into 2 L flask (or greater)		#Pour into 2 L flask (or greater)
	#Autoclave (liquid cycle)		#Autoclave (liquid cycle)
		+	#Allow liquid to cool to less than 60 degrees Centrigrade
	#Add 100 mL [[potassium phosphate solution]]		#Add 100 mL [[potassium phosphate solution]]

CnorthMSU at 15:55, 14 December 2006

CnorthMSU — Thu, 14 Dec 2006 15:55:58 GMT

←Older revision		Revision as of 15:55, 14 December 2006
Line 11:		Line 11:
	#Pour into 2 L flask (or greater)		#Pour into 2 L flask (or greater)
	#Autoclave (liquid cycle)		#Autoclave (liquid cycle)
-	#Add 100 mL potassium phosphate solution	+	#Add 100 mL [[potassium phosphate solution]]

Dstrick: /* Ingredients */

Dstrick — Wed, 26 Apr 2006 15:10:02 GMT

Ingredients

←Older revision		Revision as of 15:10, 26 April 2006
Line 5:		Line 5:
	#24 g Bacto yeast extract		#24 g Bacto yeast extract
	#4 mL Glycerol		#4 mL Glycerol
-
	#100 mL 0.17M KH2PO4 and 0.72M K2HPO4, sterile, prepared separately		#100 mL 0.17M KH2PO4 and 0.72M K2HPO4, sterile, prepared separately

Dstrick at 15:09, 26 April 2006

Dstrick — Wed, 26 Apr 2006 15:09:42 GMT

New page

== Summary ==
Terrific broth (TB, not to be confused with T Broth)
== Ingredients ==
#12 g Bacto tryptone
#24 g Bacto yeast extract
#4 mL Glycerol

#100 mL 0.17M KH2PO4 and 0.72M K2HPO4, sterile, prepared separately

==Protocol==
#Mix tryptone, yeast extract and glycerol, and add distilled water up to 900mL
#Pour into 2 L flask (or greater)
#Autoclave (liquid cycle)
#Add 100 mL potassium phosphate solution