Terrific Broth: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(Rephrasing of potassium phosphate solution for better clarity) |
(added tags) |
||
Line 13: | Line 13: | ||
#Allow liquid to cool to less than 60 degrees Centrigrade | #Allow liquid to cool to less than 60 degrees Centrigrade | ||
#Add 100 mL [[potassium phosphate solution]] | #Add 100 mL [[potassium phosphate solution]] | ||
[[Category:Material]] [[Category:Standard Media]] |
Latest revision as of 14:13, 11 March 2008
Summary
Terrific broth (TB, not to be confused with T Broth)
Ingredients
- 12 g Bacto tryptone
- 24 g Bacto yeast extract
- 4 mL Glycerol
- 100 mL 0.17M KH2PO4 and 0.72M K2HPO4, sterile, to be prepared separately from the tryptone, yeast extract, and glycerol solution.
Protocol
- Mix tryptone, yeast extract and glycerol, and add distilled water up to 900mL
- Pour into 2 L flask (or greater)
- Autoclave (liquid cycle)
- Allow liquid to cool to less than 60 degrees Centrigrade
- Add 100 mL potassium phosphate solution