http://openwetware.org/index.php?title=Terminal_Transferase&feed=atom&action=history 2013-05-25T08:21:17Z Revision history for this page on the wiki MediaWiki 1.13.2 http://openwetware.org/index.php?title=Terminal_Transferase&diff=118316&oldid=prev 2007-05-24T14:24:00Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 14:24, 24 May 2007</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 10:</td> <td colspan="2" class="diff-lineno">Line 10:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Links==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Links==</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.neb.com/nebecomm/products/productM0252.asp</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.neb.com/nebecomm/products/productM0252.asp</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Category:Material]] [[Category:Enzyme]] [[Category:Polymerase]] [[Category:DNA]]</ins></div></td></tr> <!-- diff generator: internal 2013-05-25 08:21:17 --> </table>

Reshma P. Shetty http://openwetware.org/index.php?title=Terminal_Transferase&diff=25656&oldid=prev 2006-03-14T00:41:44Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 00:41, 14 March 2006</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{{stub}}</ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Terminal Transferase (TdT) adds any deoxynucleotides in a template-independent manner to the 3' end of DNA.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Terminal Transferase (TdT) adds any deoxynucleotides in a template-independent manner to the 3' end of DNA.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <!-- diff generator: internal 2013-05-25 08:21:17 --> </table>

Austin J. Che http://openwetware.org/index.php?title=Terminal_Transferase&diff=25650&oldid=prev 2006-03-14T00:35:58Z

<p></p> <p><b>New page</b></p><div>Terminal Transferase (TdT) adds any deoxynucleotides in a template-independent manner to the 3' end of DNA.<br /> <br /> People usually use this to poly-tail with a certain nucleotide. According to NEB's site, with an excess of a single nucleotide, the tail lengths with TdT after 15 minutes are dA: 300-500, dC: 100-150, dG: 15-25, dT: 250-350<br /> <br /> However, here's some anecdotal evidence I have of nucleotide distribution: I used TdT for 30 minutes @37C in NEB4+CoCl2, with all 4 nucleotides (0.25mM each). Of 4 colonies sequenced, the random sequences were of lengths: 133, 198, 42, 69. The stats of the sequence generated was: 132 A, 54 T, 81 C, 175 G (total 442 nt). This shows a heavy G-bias which doesn't seem to agree with NEB. --[[User:Austin|Austin]] 19:35, 13 March 2006 (EST)<br /> <br /> ==Links==<br /> http://www.neb.com/nebecomm/products/productM0252.asp</div>

Austin J. Che