TempliPhi

From OpenWetWare

Revision as of 11:54, 2 January 2007 by Reshma P. Shetty (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

Contents

Overview

A kit for in vitro amplification of plasmid DNA for sequencing. It enables sequencing direct from a single colony thereby avoiding overnight cultures and minipreps. It is also useful for sequencing low copy plasmids which give poor yields from minipreps. This kit is based on the φ29 DNA polymerase and uses rolling circle amplification.

Materials

Specific protocols

References

  1. Dean FB, Nelson JR, Giesler TL, and Lasken RS. . pmid:11381035. PubMed HubMed [Dean-GenomeRes-2001]
  2. Reagin MJ, Giesler TL, Merla AL, Resetar-Gerke JM, Kapolka KM, and Mamone JA. . pmid:14676313. PubMed HubMed [Reagin-JBiomolTech-2003]
  3. TempliPhi FAQ

    [FAQ]

All Medline abstracts: PubMed HubMed
Personal tools