Reshma P. Shetty at 16:54, 2 January 2007

Reshma P. Shetty — Tue, 02 Jan 2007 16:54:54 GMT

←Older revision		Revision as of 16:54, 2 January 2007
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		+	==Overview==
		+	A kit for in vitro amplification of plasmid DNA for sequencing. It enables sequencing direct from a single colony thereby avoiding overnight cultures and minipreps. It is also useful for sequencing low copy plasmids which give poor yields from minipreps. This kit is based on the φ29 DNA polymerase and uses [http://www6.amershambiosciences.com/aptrix/upp01077.nsf/Content/autodna_templiphi_rollingcircle rolling circle amplification].
		+
	==Materials==		==Materials==
	*[http://www4.amershambiosciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&parentid=25640010&moduleid=164891 illustra TempliPhi™ 100/500 Amplification Kit]		*[http://www4.amershambiosciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&parentid=25640010&moduleid=164891 illustra TempliPhi™ 100/500 Amplification Kit]

	==Specific protocols==		==Specific protocols==
-	[[Endy:Templiphi]]	+	*[[Endy:Templiphi]]
		+	*[[Knight:TempliPhi]]

		+	==References==
		+	<biblio>
		+	#Dean-GenomeRes-2001 pmid=11381035
		+	#Reagin-JBiomolTech-2003 pmid=14676313
		+	#FAQ [http://www6.amershambiosciences.com/aptrix/upp01077.nsf/Content/autodna_templiphi_faq TempliPhi FAQ]
		+	</biblio>

	[[Category:In vitro]]		[[Category:In vitro]]
	[[Category:Protocol]]		[[Category:Protocol]]
	[[Category:DNA]]		[[Category:DNA]]

Reshma P. Shetty at 20:04, 10 December 2006

Reshma P. Shetty — Sun, 10 Dec 2006 20:04:00 GMT

New page

==Materials==
*[http://www4.amershambiosciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&parentid=25640010&moduleid=164891 illustra TempliPhi™ 100/500 Amplification Kit]

==Specific protocols==
[[Endy:Templiphi]]

[[Category:In vitro]]
[[Category:Protocol]]
[[Category:DNA]]

TempliPhi - Revision history

Reshma P. Shetty at 16:54, 2 January 2007

Reshma P. Shetty at 20:04, 10 December 2006