TempliPhi

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Current revision (11:54, 2 January 2007) (view source)
 
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==Overview==
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A kit for in vitro amplification of plasmid DNA for sequencing.  It enables sequencing direct from a single colony thereby avoiding overnight cultures and minipreps.  It is also useful for sequencing low copy plasmids which give poor yields from minipreps.  This kit is based on the φ29 DNA polymerase and uses [http://www6.amershambiosciences.com/aptrix/upp01077.nsf/Content/autodna_templiphi_rollingcircle rolling circle amplification].
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==Materials==
==Materials==
*[http://www4.amershambiosciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&parentid=25640010&moduleid=164891 illustra TempliPhi™ 100/500 Amplification Kit]
*[http://www4.amershambiosciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&parentid=25640010&moduleid=164891 illustra TempliPhi™ 100/500 Amplification Kit]
==Specific protocols==
==Specific protocols==
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[[Endy:Templiphi]]
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*[[Endy:Templiphi]]
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*[[Knight:TempliPhi]]
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==References==
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<biblio>
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#Dean-GenomeRes-2001 pmid=11381035
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#Reagin-JBiomolTech-2003 pmid=14676313
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#FAQ [http://www6.amershambiosciences.com/aptrix/upp01077.nsf/Content/autodna_templiphi_faq TempliPhi FAQ]
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</biblio>
[[Category:In vitro]]
[[Category:In vitro]]
[[Category:Protocol]]
[[Category:Protocol]]
[[Category:DNA]]
[[Category:DNA]]

Current revision

Contents

Overview

A kit for in vitro amplification of plasmid DNA for sequencing. It enables sequencing direct from a single colony thereby avoiding overnight cultures and minipreps. It is also useful for sequencing low copy plasmids which give poor yields from minipreps. This kit is based on the φ29 DNA polymerase and uses rolling circle amplification.

Materials

Specific protocols

References

  1. Dean FB, Nelson JR, Giesler TL, and Lasken RS. . pmid:11381035. PubMed HubMed [Dean-GenomeRes-2001]
  2. Reagin MJ, Giesler TL, Merla AL, Resetar-Gerke JM, Kapolka KM, and Mamone JA. . pmid:14676313. PubMed HubMed [Reagin-JBiomolTech-2003]
  3. TempliPhi FAQ [FAQ]
All Medline abstracts: PubMed HubMed
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