TempliPhi
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| + | ==Overview== | ||
| + | A kit for in vitro amplification of plasmid DNA for sequencing. It enables sequencing direct from a single colony thereby avoiding overnight cultures and minipreps. It is also useful for sequencing low copy plasmids which give poor yields from minipreps. This kit is based on the φ29 DNA polymerase and uses [http://www6.amershambiosciences.com/aptrix/upp01077.nsf/Content/autodna_templiphi_rollingcircle rolling circle amplification]. | ||
| + | |||
==Materials== | ==Materials== | ||
*[http://www4.amershambiosciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&parentid=25640010&moduleid=164891 illustra TempliPhi™ 100/500 Amplification Kit] | *[http://www4.amershambiosciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&parentid=25640010&moduleid=164891 illustra TempliPhi™ 100/500 Amplification Kit] | ||
==Specific protocols== | ==Specific protocols== | ||
| - | [[Endy:Templiphi]] | + | *[[Endy:Templiphi]] |
| + | *[[Knight:TempliPhi]] | ||
| + | ==References== | ||
| + | <biblio> | ||
| + | #Dean-GenomeRes-2001 pmid=11381035 | ||
| + | #Reagin-JBiomolTech-2003 pmid=14676313 | ||
| + | #FAQ [http://www6.amershambiosciences.com/aptrix/upp01077.nsf/Content/autodna_templiphi_faq TempliPhi FAQ] | ||
| + | </biblio> | ||
[[Category:In vitro]] | [[Category:In vitro]] | ||
[[Category:Protocol]] | [[Category:Protocol]] | ||
[[Category:DNA]] | [[Category:DNA]] | ||
Current revision
Contents |
Overview
A kit for in vitro amplification of plasmid DNA for sequencing. It enables sequencing direct from a single colony thereby avoiding overnight cultures and minipreps. It is also useful for sequencing low copy plasmids which give poor yields from minipreps. This kit is based on the φ29 DNA polymerase and uses rolling circle amplification.
