Template:UCR/Breaking-RNA/HEAD: Difference between revisions
No edit summary |
No edit summary |
||
| Line 45: | Line 45: | ||
{ | { | ||
font-size: 20px; | font-size: 20px; | ||
position: relative; | position: relative; | ||
text-align:center; | text-align:center; | ||
| Line 61: | Line 60: | ||
#objectives ul | #objectives ul | ||
{ | { | ||
padding: | padding: 0 0 0 30%; | ||
margin: 0 auto; | |||
} | } | ||
#objectives li | #objectives li | ||
| Line 257: | Line 257: | ||
<li>iii) Assembly of the circuits. </li> | <li>iii) Assembly of the circuits. </li> | ||
</ul>--> | </ul>--> | ||
<!--<div id='objectives'> | |||
<h3>Engineering biological circuits necessitates the integration of three crucial components:</h3> | |||
<ul> | |||
<li> 1) Modeling the candidate clock and switch architectures. </li> | |||
<li> 2) Aptamer and Kleptamer Design </li> | |||
<li> 3) Assembly of the circuits. </li> | |||
</ul> | |||
</div>--> | |||
</body> | </body> | ||
</html> | </html> | ||
Revision as of 23:37, 20 October 2014
UNDER CONSTRUCTION
<html lang=""> <head>
<meta charset='utf-8'> <meta http-equiv="X-UA-Compatible" content="IE=edge"> <meta name="viewport" content="width=device-width, initial-scale=1"> <link rel="stylesheet" href="styles.css"> <script src="http://code.jquery.com/jquery-latest.min.js" type="text/javascript"></script> <script src="script.js"></script> <title>Breaking RNA</title>
<style>
- column-one,.toc {
display: none;
} body.mediawiki {
background: url(http://openwetware.org/images/8/8c/Breaking_RNA_Background.png); background-size: cover;
}
- globalWrapper{
width: 80%; margin-left: 10%; position: absolute;
}
- content{
margin: 0px;
<!-- background: #e6e6e6; -->
background: white;
}
h1,h2,h3,p,ul,li {
font-family: 'Open Sans', sans-serif; font-weight: 300; color: Black; text-align: justify;
} h1
{
text-align: center;
}
h3
{
font-size: 20px;
position: relative;
text-align:center;
}
p
{
font-size: 16px;
line-height: 30px;
padding: 10px 150px;
}
- objectives
{
}
- objectives ul
{
padding: 0 0 0 30%;
margin: 0 auto;
}
- objectives li
{
font-size: 18px;
list-style: none;
line-height: 30px;
}
- submenu
{
margin: 0px 40px 0px 22px;
}
- submenu a
{
color: rgba(255,255,255,1);
text-decoration: underline;
}
- submenu ul
{
text-align: center;
padding: 8px 0px;
border: 3px solid black;
background: url(http://openwetware.org/images/8/8c/Breaking_RNA_Background.png);
background-size: cover;
}
- submenu li
{
font-size: 18px;
color: white;
padding: 0px 20px;
display: inline;
}
@import url(http://fonts.googleapis.com/css?family=Open+Sans:700);
#cssmenu {
background: url(http://openwetware.org/images/8/8c/Breaking_RNA_Background.png); background-size: cover; width: auto; border: 3px solid rgba(0,0,0,0.8);
} #cssmenu ul { list-style: none; margin: 0; padding: 0; line-height: 1;
text-align:center;
display: block; zoom: 1; } #cssmenu ul:after { content: " "; display: block; font-size: 0; height: 0; clear: both; visibility: hidden; } #cssmenu ul li { display: inline-block; padding: 0; margin: 0;
line-height: 1;
} #cssmenu.align-right ul li { float: right; } #cssmenu.align-center ul { text-align: center; } #cssmenu ul li a { color: #ffffff; text-decoration: none; display: block; padding: 15px 25px; font-family: 'Open Sans', sans-serif; font-weight: 300; text-transform: uppercase; font-size: 14px; position: relative; -webkit-transition: color .25s; -moz-transition: color .25s; -ms-transition: color .25s; -o-transition: color .25s; transition: color .25s; } #cssmenu ul li a:hover { color: #dd1a1a; } #cssmenu ul li a:hover:before { width: 100%; } #cssmenu ul li a:after { content: ""; display: block; position: absolute; right: -3px; top: 19px; height: 6px; width: 6px; background: #ffffff; opacity: .5; } #cssmenu ul li a:before { content: ""; display: block; position: absolute; left: 0; bottom: 0; height: 3px; width: 0; background: #c91616; -webkit-transition: width .25s; -moz-transition: width .25s; -ms-transition: width .25s; -o-transition: width .25s; transition: width .25s; } #cssmenu ul li.last > a:after, #cssmenu ul li:last-child > a:after { display: none; } #cssmenu ul li.active a { color: #aaaaaa; } #cssmenu ul li.active a:before { width: 100%; } #cssmenu.align-right li.last > a:after, #cssmenu.align-right li:last-child > a:after { display: block; } #cssmenu.align-right li:first-child a:after { display: none; } @media screen and (max-width: 768px) { #cssmenu ul li { float: none; display: block; } #cssmenu ul li a { width: 100%; -moz-box-sizing: border-box; -webkit-box-sizing: border-box; box-sizing: border-box; border-bottom: 1px solid #ffffff; } #cssmenu ul li.last > a, #cssmenu ul li:last-child > a { border: 0; } #cssmenu ul li a:after { display: none; } #cssmenu ul li a:before { display: none; } }
</style> </head> <body>
<!--<img src="http://openwetware.org/images/4/4e/Bistable_Ribose_Logo.png" >--> <!--<img src="http://openwetware.org/images/thumb/4/4e/Bistable_Ribose_Logo.png/800px-Bistable_Ribose_Logo.png">-->
<div id='cssmenu'> <ul>
<li><a href='http://openwetware.org/wiki/Biomod/2014/UCR/Breaking_RNA'><span>Home</span></a></li> <li><a href='http://openwetware.org/wiki/Biomod/2014/UCR/Breaking_RNA/Project'><span>Motivation & Objectives</span></a></li> <li><a href='http://openwetware.org/wiki/Biomod/2014/UCR/Breaking_RNA/Results'><span>Results</span></a></li> <li><a href='http://openwetware.org/wiki/Biomod/2014/UCR/Breaking_RNA/Methods'><span>Methods</span></a></li> <li><a href='http://openwetware.org/wiki/Biomod/2014/UCR/Breaking_RNA/Acknowledgements'><span>Supplement</span></a></li> <li class='last'><a href='http://openwetware.org/wiki/Biomod/2014/UCR/Breaking_RNA/Members'><span>Team</span></a></li>
</ul> </div> <!--<h3><u>A Synthetic Oscillatory System using Enzyme Aptamers</u></h3>
<p>In this topology, E1 and E2 are enzymes, while R1,R2,R3, and R4 represent RNA strands. Our aim is to develop an oscillatory system, where E1 would inhibit E2, and E2 would activate E1. The negative feedback loop of E1 is essential for the current model to oscillate, while the positive feedback loop may serve to improve the oscillations observed.</p> <p>Biomolecular clocks and toggle switches are circuits with important roles in biological systems; for instance, oscillators direct circadian rhythms, and bistable circuits control many developmental pathways. We are building simple synthetic transcriptional in vitro oscillatory and bistable circuits using RNA aptamers. Similar circuits have been constructed in the past; however, our systems are the first to utilize RNA aptamers. These RNA aptamers regulate bacterial RNA polymerases’ activity to generate positive and negative feedback loops. We built numerical models, which show our systems exhibit the expected behaviors. Experimentally, we have shown that RNA polymerases can be inhibited and reactivated by the binding and removal of the aptamers through strand displacement. This is an important step towards experimentally validating our circuits. The simplicity of our design allows it to be easily built upon, thus, paving the way for new biological circuits where RNA aptamers are powerful, programmable tools for designing dynamic interactions.</p>-->
<!--<li><a href='http://openwetware.org/wiki/Biomod/2014/UCR/Breaking_RNA/Achievements'><span>Achievements</span></a></li>--> <!--<h2> Content </h2>--> <!--<div id='submenu'>
<ul>
<li><a href="#">Aptamer and Complement Design</a></li>
<li>List</li>
<li>List</li>
</ul> -->
<!-- <div id='objectives'>
<ul>
<li> i) Modeling the candidate clock and switch architectures. </li>
<li> ii) Building reversible RNA pathways to inhibit and activate enzymes. </li>
<li>iii) Assembly of the circuits. </li>
</ul>-->
<!--<div id='objectives'> <h3>Engineering biological circuits necessitates the integration of three crucial components:</h3>
<ul>
<li> 1) Modeling the candidate clock and switch architectures. </li>
<li> 2) Aptamer and Kleptamer Design </li>
<li> 3) Assembly of the circuits. </li>
</ul>
</div>--> </body> </html>
