Template:SBB10 ConstructionFiles XiaoL sbb03
From OpenWetWare
Construction file for tos (check for errors)
1) sbb03: tos site of N15 Pool tos01 through tos20, assemble by PCA(550bp,gp) PCR tos01/tos20 on PCA reaction (550bp,EcoR1/BamH1,) Sub into pBca9145-jtk2625 (EcoRI/BamHI, 2057+929bp, L) Product is pBca9145-sbb03 ------------ tos01 PCA assembly of tos CCATAGAATTCATGAGATCTTCTAAGCGCAACGGTATTACTTACGT tos02 PCA assembly of tos ATTTAAAATCATTAAGTTAGGTTTTAAATATACCAACGTAAGTAATACCGTTGC tos03 PCA assembly of tos TTAAAACCTAACTTAATGATTTTAAATGATAATAAATCATACCAATTGC tos04 PCA assembly of tos CAGCATGTTCGCTTAACTTTTGATAGCAATTGGTATGATTTATTATC tos05 PCA assembly of tos AAAGTTAAGCGAACATGCTGATTTTCACGCTGTTTATACACTTTGAG tos06 PCA assembly of tos ATATAGAGACGGAAGAGATAGAGATGCCTCAAAGTGTATAAACAGCG tos07 PCA assembly of tos TATCTCTTCCGTCTCTATATTGAAACACAATCAAAGAACATCAATCC tos08 PCA assembly of tos TTCTTAGATAGTGGGGGATGTCACATGGATTGATGTTCTTTGATTG tos09 PCA assembly of tos ATCCCCCACTATCTAAGAACACCATAACAGAACACAACATAGGAATG tos10 PCA assembly of tos TGTTCCGAATTATTGATACATTAATGTTGCATTCCTATGTTGTGTTCTG tos11 PCA assembly of tos AATGTATCAATAATTCGGAACATATGCACTATATCATATCTCAATTACGG tos12 PCA assembly of tos ATGGGCAATTGTGTGCTGATATGTTCCGTAATTGAGATATGATATAG tos13 PCA assembly of tos TCAGCACACAATTGCCCATTATACGCGCGTATAATGGACTATTGTGTG tos14 PCA assembly of tos GTTCTGCGCTTATGTTCTCCTTATCAGCACACAATAGTCCATTATAC tos15 PCA assembly of tos GAGAACATAAGCGCAGAACAATATGTATCTATTCCGGTGTTGTGTTC tos16 PCA assembly of tos TATAAGAGAACATAATAGCAGAATAACAAAGGAACACAACACCGGAATAG tos17 PCA assembly of tos TCTGCTATTATGTTCTCTTATAGTGTGACGAAAGCAGCATAATTAATC tos18 PCA assembly of tos ATATCGTAACACAATCAAAGAACAAGTGACGATTAATTATGCTGCTTTCG tos19 PCA assembly of tos TTCTTTGATTGTGTTACGATATCCAGAGACTTAGAAACGGGGGAAGG tos20 PCA assembly of tos CATAGGGATCCTTCCCCCGTTTCTAAGT
JCA Notes
- correct
