Template:SBB10 ConstructionFiles JoseG sbb38: Difference between revisions
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(New page: Biobricking of Pbad PCR JG003-F and JG004-R on pBca1100-Bca1152. (276 bp, EcoRI/BamHI) Sub into pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L) Product is Pbad-pBjk2...) |
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Revision as of 23:17, 8 February 2010
Biobricking of Pbad
PCR JG003-F and JG004-R on pBca1100-Bca1152. (276 bp, EcoRI/BamHI)
Sub into pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L)
Product is Pbad-pBjk2741 {Pbad-J23100}
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JG003-F Cloning of papC ccataGAATTCgtccAGATCTaaccaattgtccatattgcatcag
JG004-R Cloning of papC ctgatGGATCCgctaatcttatggataaaaatgc
